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memory T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress®
Whole Blood CD4+
Memory T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45RO-APC, CD4-PE, CD45RA-FITC, and CD45-VioBlue®
and analyzed by flow cytometry using the MACSQuant®
Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. CD45RA versus CD45RO expression is shown for gated CD4+
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