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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
HM1 mouse embryonic stem cells carrying a GFP reporter gene were transfected with 750 ng StemMACS™ Cas9 Nickase mRNA and two plasmids encoding guide RNAs (125 ng each). Transfected (A) and untransfected cells (B) were analyzed by flow cytometry after 72 hours. 9.91% of all cells were GFP-negative indicating successful eGFP knockout.
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