Mouse M-CSF

Mouse M-CSF

Recombinant mouse M-CSF regulates the differentiation, proliferation, and survival of osteoclasts, macrophages, monocytes, and their hematopoietic progenitors. As a potent hematopoietic cytokine, macrophage colony-stimulating factor (M-CSF) is involved in diverse processes, such as regulation of atherosclerosis or brain, bone resorption, placental development, and inflammatory responses. This recombinant protein has been optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse M-CSF can be used for a variety of applications, including:
  • Generation of bone marrow-derived macrophages.
  • Differentiation of osteoclasts.
  • Proliferation and apoptosis assays.

Background information

Macrophage-colony stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Proliferation of M-NFS-60 cells
  • premium grade: ≥ 2×
    10
    5
    U/mg
  • research grade: ≥ 1×
    10
    5
    U/mg
  • Selected references

    1. Guery L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704
    2. Tanne, A. et al. (2009)
      A murine DC-SIGN homologue contributes to early host defense against
      Mycobacterium tuberculosis
      .
      J. Exp. Med. 206(10): 2205-2220
    3. Tanne, A. et al. (2009)
      A murine DC-SIGN homologue contributes to early host defense against
      Mycobacterium tuberculosis
      .
      J. Exp. Med. 206: 2205-2220
    4. Herold, J. et al. (2014) Tetanus toxoid-pulsed monocyte vaccination for augmentation of collateral vessel growth. J Am Heart Assoc. 3(2): e000611
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
EUR 160,00 
E. coli
25 µg
EUR 295,00 
E. coli
10 µg
EUR 195,00 
E. coli
25 µg
EUR 375,00 
E. coli
100 µg
EUR 645,00 
E. coli
1000 µg
EUR 2 820,00