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Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by MACS NeuroBrew-21 and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression.
TH (tyrosine hydroxylase)+
TuJ1 (neuron-specific β-III tubulin)+
GAD67 (glutamate decarboxylase)+
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