Clone:
REA1297
Type of antibody:
Primary antibodies, Recombinant antibodies, Anti-idiotype antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
Anti-FMC63 scFv Antibody

Specifications for CD19 CAR FMC63 Idiotype Antibody, REAfinity™

Overview

The CD19 CAR FMC63 Idiotype Antibody, REAfinity™ has been developed for the detection of genetically modified T cells that are engineered to express a chimeric antigen receptor (CAR) comprising a single chain variable fragment (scFv), which derives from the mouse anti-human CD19 antibody clone FMC63. The CD19 CAR FMC63 Idiotype Antibodies, REA1297 and REA1298, are anti-idiotype antibodies that recognize the FMC63 scFv and feature a specifically mutated human IgG1 Fc region. The mutated human IgG1 Fc region of the CD19 CAR FMC63 Idiotype Antibody abolishes its binding to Fcγ receptors, which allows for background-free analysis and eliminates the need for additional blocking steps, such as using a FcR blocking reagent.

Alternative names

Anti-FMC63 scFv Antibody

Detailed product information

Technical data

To compare the epitope specificity of the CD19 CAR FMC63 Idiotype Antibodies REA1297 and REA1298, competition assays with both clones and CD19 Fc fusion protein (CD19 Fc) were performed. CD19 CAR T cells were incubated with an excess of unconjugated CD19 Fc, followed by staining with fluorochrome-conjugated REA1297 or REA1298. Based on the fluorescence signal obtained,
the clones were identified as recognizing overlapping or different epitopes on the FMC63 derived scFv. CD19 Fc prevented staining with REA1297, whereas CD19 Fc and REA1298 staining did not interfere with each other. This demonstrates that REA1297 reacts with a similar site as CD19, but REA1298 binds to a different part of FMC63 scFv (outside the paratope), and hence, does not compete for the CD19 antigen-binding domain.
To compare the epitope specificity of the CD19 CAR FMC63 Idiotype Antibodies REA1297 and REA1298, competition assays with both clones and CD19 Fc fusion protein (CD19 Fc) were performed. CD19 CAR T cells were incubated with an excess of unconjugated CD19 Fc, followed by staining with fluorochrome-conjugated REA1297 or REA1298. Based on the fluorescence signal obtained,
the clones were identified as recognizing overlapping or different epitopes on the FMC63 derived scFv. CD19 Fc prevented staining with REA1297, whereas CD19 Fc and REA1298 staining did not interfere with each other. This demonstrates that REA1297 reacts with a similar site as CD19, but REA1298 binds to a different part of FMC63 scFv (outside the paratope), and hence, does not compete for the CD19 antigen-binding domain.

Technical specifications

CloneREA1297
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies, Anti-idiotype antibodies
Specieshuman
AntigenCD19 CAR
Alternative names of antigenAnti-FMC63 scFv Antibody
Distribution of antigenCAR T cells, CAR NK cells

Resources for CD19 CAR FMC63 Idiotype Antibody, REAfinity™

Documents and Protocols

Certificates

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