Order a free sample and test full flexibility in iPS and ES cell differentiation
Our xeno-free (XF) StemMACS™ DiffBase XF, human serves as optimized and standardized base medium for the differentiation of human pluripotent stem cells (hPSCs). When supplemented with the appropriate patterning cytokines and small molecules, it facilitates virtually every type of directed differentiation protocol. hPSCs cultivated in StemMACS iPS-Brew XF, human can directly start the differentiation protocol with StemMACS DiffBase XF, human without adapting the culture to the new medium formulation. Moreover, unsupplemented StemMACS DiffBase XF, human can be conveniently used to assess hPSC pluripotency by embryoid body (EB) assay.
StemMACS™ DiffBase XF, human can be used as base medium for any type of directed differentiation after supplementation with the appropriate patterning cytokines and small molecules. Here we show that cultivation in StemMACS DiffBase XF supplemented with germ-layer specific patterning factors is successful both in directed differentiation protocols based on monolayer adherent cultures (A) and in protocols based on EB intermediate 3D culture (B). In fact, after supplementation with either ectoderm-, mesoderm-, or endoderm-specific patterning factors, the culture starts to express lineage-specific differentiation markers. As demonstrated by flow cytometric analysis, expression of early differentiation markers is detected already after six days.
StemMACS DiffBase XF, human and StemMACS iPS-Brew XF, human share similar biochemical and physicochemical characteristics, which allows a smooth and gentle transition into the differentiation protocol without the need to adapt the cells to the new medium. This results in a shorter cultivation time and less stress for the cells.
Without adding cytokines, StemMACS™ DiffBase XF, human supports cell survival and multiplication and thus can be used to assess PSC pluripotency by EB assay. In fact, StemMACS DiffBase XF, human supports the formation and long-term survival of EBs (fig. 3).
EBs grown in suspension for two weeks and in adherent culture for one week using StemMACS DiffBase XF, human, differentiate into cellular types deriving from the three embryonic germ layers (fig. 4).