Isolation, characterization, and cultivation of oligodendrocytes

Realize your potential with our definitive solutions for efficient and supportive workflow for oligodendrocytes. Whether you require support in the isolation of pure pre-mature oligodendrocytes (O4 positive), oligodendrocyte precursor cells (OPCs) from neonatal brain tissue in an hour, or viable pre-mature oligodendrocytes (O4 positive) from adult brain tissue in half a day –  with our MACS technology, the solution for high yield of viable single cells is simple. Furthermore, our neonatal or adult oligodendrocyte cultivate in serum-free medium provides the opportunity for extended growth and survival of mouse oligodendrocytes, maintaining the validity of your cell lines for longer.

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Neuroscience

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Debris and erythrocytes detected in cell suspensions before and after sample clearing

Preparation of viable adult brain cells free of debris and red blood cells

Combined optimized enzymatic treatment using the Adult Brain Dissociation Kit with gentle mechanical dissociation using the gentleMACS™ Octo Dissociator with Heaters results in pure and viable single-cell suspensions. 

Purity and viability of cells obtained from murine (P4) whole brain tissue

Standardized dissociation of neonatal neural tissues

Neural Tissue Dissociation Kits allow reliable tissue dissociation and result in a high yield of viable single cells.

Single-cell suspension from P2 mouse brain before and after separation using MACS MicroBead Technology

Isolation of oligodendrocyte precursor cells

Obtain pure, viable, and functional oligodendrocyte precursor cells by positive selection with the CD140a (PDGFRα) MicroBead Kit, mouse.

Single-cell suspensions from neonatal and adult brain before and after separation using MACS MicroBead Technology

Isolation of pre-mature oligodendrocytes from neonatal or adult brain

High recovery of pre-mature oligodendrocytes from neonatal and adult brain with Anti-O4 MicroBeads, human, mouse, rat.

Scientific poster
Efficient isolation of viable primary neural cells from adult murine brain tissue based on a novel automated tissue dissociation protocol

Hui Zhang1, Sandy Reiß1, Stefan Tomiuk1, Silvia Rüberg1, Richard Fekete2, Melanie Jungblut1, and Andreas Bosio1

1Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany; 2Fluidigm Corporation, South San Francisco, CA, USA

Cultivation of isolated oligodendrocytes

Best culture conditions for oligodendrocytes

MACS® Neuro Medium supplemented with the serum-free MACS NeuroBrew®-21, PDGF-AA and FGF-2, are optimized to support growth and long-term survival of mouse oligodendrocytes.  

Anti-O4 staining of adult mouse oligodendrocytes

Anti-O4 staining of isolated oligodendrocytes 

Specific immunocytochemistry and immunohistochemistry staining of oligodenrocytes from adult mouse brain.

Get acquainted with our protocols for the isolation, characterization, and cultivation of oligodendrocytes.

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