ISCT 2020 Paris Virtual

It was great meeting you online at ISCT 2020 Paris Virtual! 

We hope to see you next year in person.  
International Society for Cell & Gene Therapy (ISCT) Annual Meeting 2021, May 26–29, New Orleans, USA 

In the meantime, watch the recorded talks of our symposium "Advanced solutions for cutting-edge therapies", check out the posters we presented virtually or join the virtual demo "Automate adherent stem cell manufacturing in a closed system".

Recorded talks

Sort-purification of human CD34+CD90+ cells reduces target cell population and improves lentiviral transduction for gene therapy

Hematopoietic stem cell (HSC) gene therapy is a promising treatment option for various hematological diseases and disorders. Most currently available approaches target CD34+ cell–enriched fractions, a heterogeneous mix of mostly committed progenitor cells and only very few true HSCs with long-term multilineage engraftment potential. Consequently, gene therapy approaches are limited in their HSC targeting efficiency and very expensive due to the large quantities of modifying reagents needed. In addition, exposure of non-target cells to lentiviral vectors or nucleases can increase the risk of unwanted side-effects in these cell populations.
We aimed to develop a clinical protocol to reliably purify and efficiently gene-modify human HSC-enriched CD90+ cell fractions, which ultimately would allow us to reduce costs without compromising in vivo engraftment.
Large-scale enrichment of CD34+ cells from GCSF-mobilized leukapheresis products was initially performed on Miltenyi Biotec’s CliniMACS Prodigy®. Yield, purity, quality, and feasibility of CD90+ cell sorting was then tested on the jet-in-air sorter FX500 from Sony and the cartridge-based closed-system sorter MACSQuant® Tyto® from Miltenyi Biotec. Transduction was performed using a clinical-grade, GFP-encoding lentivirus. Engraftment was tested using the NSG mouse xenograft model.
Purity and yield after flow cytometric sorting of CD90+ cells were similar with either the FX500 or MACSQuant® Tyto®. Both approaches reliably reduced the overall target cell count by 10- to 15-fold without impacting the cells’ viability and in vitro colony-forming cell potential. Transduction efficiency of sorted CD90+ cells was significantly improved compared to bulk CD34+ and especially the CD34+CD90+ subset. All cell fractions demonstrated robust mouse xenograft potential. Significantly higher levels of GFP expression in peripheral blood, bone marrow, spleen, and thymus were observed after transplantation of gene-modified CD90+ compared to bulk CD34+ cells in NSG mice.

Stefan Radtke 
Chairman, Interdisciplinary Society for Lab Diagnostics and Flow Cytometry Fred Hutchinson Cancer Research Center, Seattle, USA

Advanced cell therapy based on the chimeric receptor NKG2D: the NK-T cell approach 

NKG2D is a C‐type lectin‐like transmembrane activating receptor present on the surface of natural killer (NK) cells, NKT cells, CD8+TCRγδ+ T cells, and certain subsets of CD4+ T cells. Biogenesis of NKG2D ligands (NKG2DL) is stimulated in cells under stress conditions such as viral infection, cellular senescence, and tumorigenesis. NKG2DL are expressed on various tumor types including different pediatric solid and hematological malignancies, thus providing suitable targets for cancer therapy. NKG2D activation on NK cells results in cytokine secretion and exocytosis of cytotoxic granules. NKG2D is particularly relevant for cancer immunosurveillance: Interaction between NKG2DL and NKG2D receptor is essential for NK cell–mediated elimination of osteosarcoma tumor‐initiating cells. However, tumor cells can develop various immune escape strategies. Nevertheless, the use of NKG2D‐CAR on memory CD45RA T cells may overcome these limitations. We have shown that NKG2D‐CAR–expressing CD45RA T cells were cytotoxic against three osteosarcoma cell lines and 8/10 leukemia cell lines with specific lysis of over 50%. Myeloid and T‐ALL cell lines were more susceptible (specific lysis ranging from 50–78%) than B‐ALL cell lines (19–52%). NKG2D‐CAR+ memory CD45RA T cells also had considerable antitumor activity in a mouse model of human osteosarcoma, whereas non-transduced T cells were ineffective. We have developed a protocol to expand clinical-grade NKG2D‐CAR–expressing memory CD45RA T cells in a fully automated closed system, CliniMACS Prodigy®. This expansion protocol allowed us to obtain up to 2076±697 million cells with 77.8±20% NKG2D‐CAR expression and 76±10% viability. Harvested CAR T cells showed specific lysis of Jurkat cells (90±14%) and 531MII osteosarcoma cell line (31±16%). Vector copy number was ≤5 in all validations. CGH and karyotype showed no genetic alterations. Free viral particles were undetectable in the supernatants. No overexpression of MYC/TERT was found except for one validation. Endotoxins were ≤0.25 EU/mL. Automated manufacturing of clinical‐grade NKG2D‐CAR–expressing memory CD45RA T cells using the CliniMACS Prodigy® is feasible and reproducible. We plan to explore different clinical trials on pediatric diseases.

Antonio Pérez‐Martínez
Head of Pediatric Hemato-Oncology Service, University Hospital La Paz, Madrid, Spain. 
University Autonoma of Madrid

Manufacturing of induced pluripotent stem cells and immune-derived cells

The breakthrough discovery of induced pluripotent stem cells (iPSCs) is currently profoundly modifying the landscape of cell therapy and allows us to open novel perspectives for the generation of advanced therapy medicinal products (ATMP) potentially applicable in all fields of medicine. In terms of a large-scale therapeutic landscape, implementation of iPSC-derived allogeneic therapies will require suitable immune-HLA-matched iPSC lines from healthy universal donors and/or the derivation of hypoimmunogenic iPSC lines. To anticipate future demands in effective allogeneic therapies, the availability of accessible cost-effective and safe iPSC lines is a major requirement, to provide off-the-shelf unlimited numbers of therapeutic products from a single iPSC master cell bank. We have previously developed several research-grade master banks of human iPSCs using an in vitro expansion workflow. However, several hurdles remain for industrial, cGMP-grade, large-scale production and banking of these cells.
We report a procedure using the integrated GMP-compliant cell processing platform CliniMACS Prodigy® providing automated cell feeding and harvesting in a closed system. The iPSC clone used on the CliniMACS Prodigy Platform was previously derived from a healthy donor using the CytoTune™- iPS 2.0 Sendai Reprogramming Kit, manufactured according to GMP principles, on StemMACS™ iPS-Brew XF, human medium and human recombinant laminin matrix. Over 14 in vitro cell passages, this cell line was replated and expanded on the CliniMACS Prodigy Platform within 2 weeks. At the end of the process, 1.4 billion iPSCs were collected with a high genetic stability as evaluated by karyotyping and CNV/NGS analysis before and after scalable expansion. Expanded iPSCs maintained their pluripotency markers and an efficient differentiation towards endodermal, mesodermal, and ectodermal trilineage layers. Overall, this process provides a safe and standardized scalable manufacturing platform for GMP-iPSC master cell banks. The feasibility of this procedure opens novel perspectives in large-scale production of mesenchymal stem cells and immunocompetent cells for our therapeutic program.

Annelise Bennaceur-Griscelli
Professor Medicine in Hematology,
University Paris Saclay, APHP– CITHERA Inserm UMR 935, Paris, France

Poster session

Posters       

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Fully automated integrated process for depletion of T cells with or without subsequent purification of NK cells from apheresis using the CliniMACS® Prodigy
Category: Immunotherapy
Poster ID: 418

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Developing a GMP-compliant, automated process to generate CAR NK cells in a closed system for clinical use
Category: Tools for genetic engineering
Poster ID: 434

Virtual demo

Learn how to automate adherent stem cell manufacturing in a closed system.

Related information
 

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