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Human mesenchymal stem cells (MSC), harvested and cultured from normal bone marrow, were fixed and permeabilized using the Cell Signaling Buffer Set A. Cells were then stained with Anti-Vimentin antibodies or with the corresponding REA Control (I) antibodies (left peak) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris were excluded from the analysis based on scatter signals.
3D-immunofluorescence analysis of human colorectal cancer fixed with 4% PFA and subjected to whole-mount immunostaining with Vimentin Antibody, anti-human, Vio R667 (red). The sample was processed and optically cleared using the MACS® Clearing Kit and imaged with an UltraMicroscope Blaze light sheet instrument. Sample detail image (A), overview image (B), 3D- rendered maximum intensity projection (C, counterstaining with EpCAM in yellow) and 3D-rendered video (D, counterstaining with EpCAM in yellow). Scale bars: 200 µm (A), 500 µm (B), 300µm (C).
Clone | REA409 |
---|---|
Clonality | monoclonal |
Isotype | recombinant human IgG1 |
Isotype control | REA Control Antibody (I), human IgG1 |
Host | human cell line |
Type of antibody | Primary antibodies, Recombinant antibodies |
Species | human |
Antigen | Vimentin |
Alternative names of antigen | VIM, CTRCT30, HEL113 |
Molecular mass of antigen [kDa] | 54 |
Distribution of antigen | Sertoli cells, endothelial cells, fibroblasts, macrophages, neutrophils, pancreas |
Entrez Gene ID | 7431 |
RRID | AB_2727581, AB_2751530, AB_2751494, AB_2889796, AB_2889795, AB_2905457, AB_2905456, AB_2727645 |
Clone | REA409 |
---|---|
Clonality | monoclonal |
Isotype | recombinant human IgG1 |
Isotype control | REA Control Antibody (I), human IgG1 |
Host | human cell line |
Type of antibody | Primary antibodies, Recombinant antibodies |
Species | human |
Antigen | Vimentin |
Alternative names of antigen | VIM, CTRCT30, HEL113 |
Molecular mass of antigen [kDa] | 54 |
Distribution of antigen | Sertoli cells, endothelial cells, fibroblasts, macrophages, neutrophils, pancreas |
Entrez Gene ID | 7431 |
RRID | AB_2727581, AB_2751530, AB_2751494, AB_2889796, AB_2889795, AB_2905457, AB_2905456, AB_2727645 |
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