The Tumor Cell Isolation Kit, mouse enables the enrichment of mouse tumor cells from primary mouse specimens using MACS
®
Cell Separation technology.
While depleting tumor associated cells such as lymphocyte subpopulations, fibroblasts, and endothelial cells in a fast and easy protocol, the tumor cells remain untouched, improving any downstream analysis.

Data and images for Tumor Cell Isolation Kit, mouse

Figures

Figure 1

Mouse breast carcinoma cells were purified from a mouse 4T1 tumor using the Tumor Cell Isolation Kit, mouse, an LS Column, and a QuadroMACS™ Separator. The 4T1 tumor cells were GFP-labeled prior to tumor induction for subsequent detection. After tumor dissociation and sorting, the unseparated fraction and the negative fraction were analyzed by flow cytometry for GFP and lineage markers (CD45, Ter119, and CD31 combined in the APC channel) using the MACSQuant
®
Analyzer.
Before separation
Isolated mouse tumor cells
View details

Figure 1

Mouse breast carcinoma cells were purified from a mouse 4T1 tumor using the Tumor Cell Isolation Kit, mouse, an LS Column, and a QuadroMACS™ Separator. The 4T1 tumor cells were GFP-labeled prior to tumor induction for subsequent detection. After tumor dissociation and sorting, the unseparated fraction and the negative fraction were analyzed by flow cytometry for GFP and lineage markers (CD45, Ter119, and CD31 combined in the APC channel) using the MACSQuant
®
Analyzer.
View details

Figure 1

Mouse breast carcinoma cells were purified from a mouse 4T1 tumor using the Tumor Cell Isolation Kit, mouse, an LS Column, and a QuadroMACS™ Separator. The 4T1 tumor cells were GFP-labeled prior to tumor induction for subsequent detection. After tumor dissociation and sorting, the unseparated fraction and the negative fraction were analyzed by flow cytometry for GFP and lineage markers (CD45, Ter119, and CD31 combined in the APC channel) using the MACSQuant
®
Analyzer.

Figure 2

GFP-expressing mouse colon carcinoma cells (CT26.WT) were isolated from a heterogenous cell population and subsequently, the unseparated fraction and the mouse tumor cell fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using CD90.2 antibody (red) and cell nuclei were stained with DAPI (blue). The tumor cells were detected by their expression of GFP (green).
Before separation
Isolated mouse tumor cells
View details

Figure 2

GFP-expressing mouse colon carcinoma cells (CT26.WT) were isolated from a heterogenous cell population and subsequently, the unseparated fraction and the mouse tumor cell fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using CD90.2 antibody (red) and cell nuclei were stained with DAPI (blue). The tumor cells were detected by their expression of GFP (green).
View details

Figure 2

GFP-expressing mouse colon carcinoma cells (CT26.WT) were isolated from a heterogenous cell population and subsequently, the unseparated fraction and the mouse tumor cell fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using CD90.2 antibody (red) and cell nuclei were stained with DAPI (blue). The tumor cells were detected by their expression of GFP (green).

Specifications for Tumor Cell Isolation Kit, mouse

Overview

The Tumor Cell Isolation Kit, mouse enables the enrichment of mouse tumor cells from primary mouse specimens using MACS
®
Cell Separation technology.
While depleting tumor associated cells such as lymphocyte subpopulations, fibroblasts, and endothelial cells in a fast and easy protocol, the tumor cells remain untouched, improving any downstream analysis.

Detailed product information

The Tumor Cell Isolation Kit, mouse has been designed for the enrichment of untouched mouse tumor cells from mouse tumors.
During the growth phase
in vivo
, tumor tissue is vascularized and infiltrated by cells of non-tumor origin, including heterogeneous lymphocyte subpopulations, fibroblasts, and endothelial cells.
The level of infiltration is highly dependent on multiple factors like tumor subtype, growth rate, site of tumor growth and status of the host animal. However, even when these factors are kept constant, the amount and composition of infiltrating cells remain widely unpredictable. Molecular downstream analysis as well as culture of mouse tumor cells is frequently challenged by non-tumor cells. Additionally, there is a lack of markers exclusively expressed on mouse tumor cells, which makes direct isolation or identification in analyses like flow cytometry difficult.
For optimal results, the Tumor Cell Isolation Kit, mouse should be used in combination with the Tumor Dissociation Kit, mouse (# 130-096-730) and gentleMACS™ Dissociators.

Columns

LS Columns, autoMACS
®
Columns, or Multi-24 Column Blocks.

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