The Tumor-Associated Fibroblast Isolation Kit, mouse has been designed for the isolation of tissue-derived, tumor-associated fibroblasts from dissociated mouse tumors.

Data and images for Tumor-Associated Fibroblast Isolation Kit, mouse

Figures

Figure 1

Mouse tumor-associated fibroblasts were purified from a mouse B16-F10 tumor (melanoma) using the Tumor-Associated Fibroblast Isolation Kit, mouse. For pre-enrichment an LD Column and a QuadroMACS™ Separator were used, subsequent isolation was performed on a MS Column and a MiniMACS™ Separator. Cells were fluorescently stained with CD45-PE-Vio
®
770 and CD90.2-APC, and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
Pre-enriched tumor-associated fibroblasts
Isolated tumor-associated fibroblasts
View details

Figure 1

Mouse tumor-associated fibroblasts were purified from a mouse B16-F10 tumor (melanoma) using the Tumor-Associated Fibroblast Isolation Kit, mouse. For pre-enrichment an LD Column and a QuadroMACS™ Separator were used, subsequent isolation was performed on a MS Column and a MiniMACS™ Separator. Cells were fluorescently stained with CD45-PE-Vio
®
770 and CD90.2-APC, and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Mouse tumor-associated fibroblasts were purified from a mouse B16-F10 tumor (melanoma) using the Tumor-Associated Fibroblast Isolation Kit, mouse. For pre-enrichment an LD Column and a QuadroMACS™ Separator were used, subsequent isolation was performed on a MS Column and a MiniMACS™ Separator. Cells were fluorescently stained with CD45-PE-Vio
®
770 and CD90.2-APC, and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Mouse tumor-associated fibroblasts were purified from a mouse B16-F10 tumor (melanoma) using the Tumor-Associated Fibroblast Isolation Kit, mouse. For pre-enrichment an LD Column and a QuadroMACS™ Separator were used, subsequent isolation was performed on a MS Column and a MiniMACS™ Separator. Cells were fluorescently stained with CD45-PE-Vio
®
770 and CD90.2-APC, and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Tumor-Associated Fibroblast Isolation Kit, mouse

Overview

The Tumor-Associated Fibroblast Isolation Kit, mouse has been designed for the isolation of tissue-derived, tumor-associated fibroblasts from dissociated mouse tumors.

Detailed product information

Background information

The Tumor-Associated Fibroblast Isolation Kit, mouse enables the isolation of tissue-derived, tumor-associated fibroblasts from dissociated mouse tumors.
Tumor-associated fibroblasts have been shown to play an important role in tumor development, progression, and function. However, they may only make up about 0.5 – 5% of the total number of cells found in tumors. Applying a two-step strategy, tumor-associated fibroblasts are first pre-enriched by removing non-target cells, followed by isolation using the general fibroblast marker CD90.2. This allows reliable isolation from different syngeneic mouse tumor models.
For optimal results, the Tumor-Associated Fibroblast Isolation Kit, mouse should be used in combination with the Tumor Dissociation Kit, mouse (# 130-096-730) and the gentleMACS™ Dissociators.

Columns

MS, LD, or autoMACS
®
Columns.

References for Tumor-Associated Fibroblast Isolation Kit, mouse

Publications

  1. Agorku, D.J. et al. (2019) CD49b, CD87, and CD95 Are Markers for Activated Cancer-Associated Fibroblasts Whereas CD39 Marks Quiescent Normal Fibroblasts in Murine Tumor Models. Front. Oncol. 9: 716

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