The Treg Detection Kit, human has been developed for the convenient detection of CD45
+
CD4
+
CD25
+
CD127
dim/neg
FoxP3
+
regulatory T (Treg) cells. The kit includes all required reagents for the analysis of human Treg cells by flow cytometry.

Data and images for Treg Detection Kit, human

Figures

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD45-VioBlue
®
, CD4-VioGreen™, CD25-VioBright™ 515, and CD127-PE antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3-Vio
®
667. Cells were analyzed by flow cytometry. Lymphocytes expressing CD45 were gated as P1 (A). From P1 gated population, autofluorescent cell debris are excluded as P2. (B). From P2, CD4-expressing cells are gated as P3 (C). From P3 gated population, Treg cells are shown using different gates that compare CD25, CD127, and FoxP3 expression (D–F).
A:
B:
View details

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD45-VioBlue
®
, CD4-VioGreen™, CD25-VioBright™ 515, and CD127-PE antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3-Vio
®
667. Cells were analyzed by flow cytometry. Lymphocytes expressing CD45 were gated as P1 (A). From P1 gated population, autofluorescent cell debris are excluded as P2. (B). From P2, CD4-expressing cells are gated as P3 (C). From P3 gated population, Treg cells are shown using different gates that compare CD25, CD127, and FoxP3 expression (D–F).
View details

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD45-VioBlue
®
, CD4-VioGreen™, CD25-VioBright™ 515, and CD127-PE antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3-Vio
®
667. Cells were analyzed by flow cytometry. Lymphocytes expressing CD45 were gated as P1 (A). From P1 gated population, autofluorescent cell debris are excluded as P2. (B). From P2, CD4-expressing cells are gated as P3 (C). From P3 gated population, Treg cells are shown using different gates that compare CD25, CD127, and FoxP3 expression (D–F).
C:
D:
View details

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD45-VioBlue
®
, CD4-VioGreen™, CD25-VioBright™ 515, and CD127-PE antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3-Vio
®
667. Cells were analyzed by flow cytometry. Lymphocytes expressing CD45 were gated as P1 (A). From P1 gated population, autofluorescent cell debris are excluded as P2. (B). From P2, CD4-expressing cells are gated as P3 (C). From P3 gated population, Treg cells are shown using different gates that compare CD25, CD127, and FoxP3 expression (D–F).
View details

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD45-VioBlue
®
, CD4-VioGreen™, CD25-VioBright™ 515, and CD127-PE antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3-Vio
®
667. Cells were analyzed by flow cytometry. Lymphocytes expressing CD45 were gated as P1 (A). From P1 gated population, autofluorescent cell debris are excluded as P2. (B). From P2, CD4-expressing cells are gated as P3 (C). From P3 gated population, Treg cells are shown using different gates that compare CD25, CD127, and FoxP3 expression (D–F).
E:
F:
View details

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD45-VioBlue
®
, CD4-VioGreen™, CD25-VioBright™ 515, and CD127-PE antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3-Vio
®
667. Cells were analyzed by flow cytometry. Lymphocytes expressing CD45 were gated as P1 (A). From P1 gated population, autofluorescent cell debris are excluded as P2. (B). From P2, CD4-expressing cells are gated as P3 (C). From P3 gated population, Treg cells are shown using different gates that compare CD25, CD127, and FoxP3 expression (D–F).
View details

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD45-VioBlue
®
, CD4-VioGreen™, CD25-VioBright™ 515, and CD127-PE antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3-Vio
®
667. Cells were analyzed by flow cytometry. Lymphocytes expressing CD45 were gated as P1 (A). From P1 gated population, autofluorescent cell debris are excluded as P2. (B). From P2, CD4-expressing cells are gated as P3 (C). From P3 gated population, Treg cells are shown using different gates that compare CD25, CD127, and FoxP3 expression (D–F).

Specifications for Treg Detection Kit, human

Overview

The Treg Detection Kit, human has been developed for the convenient detection of CD45
+
CD4
+
CD25
+
CD127
dim/neg
FoxP3
+
regulatory T (Treg) cells. The kit includes all required reagents for the analysis of human Treg cells by flow cytometry.

Detailed product information

Background information

Treg cells are a subset of T cells with the ability to suppress harmful immunological reactions to self and foreign antigens. Due to the lack of a specific marker for Treg cells, a combination of CD4, CD25, CD127, and anti-FoxP3 is commonly used for their identification. The Treg Detection Kit includes all reagents necessary for cell surface staining of CD45, CD4, CD25, and CD127, and intracellular staining of FoxP3 with a special FoxP3 Staining Buffer Set. The kit also includes Tandem Signal Enhancer and an optimized protocol.

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