The Treg Detection Kit (CD4/CD25/CD127) has been developed as a simple one-reagent solution for the identification of human regulatory T (Treg) cells by flow cytometry. Applications include
  • Staining and enumeration of CD4+CD25+CD127dim/– regulatory T cells in PBMCs, apheresis products, umbilical cord blood, or whole blood
  • Analysis of Treg cells separated using MACS® Technology

Data and images for Treg Detection Kit (CD4/CD25/CD127), human

Figures

Figure 1

Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4
+
CD25
high
CD127
dim/
cells.
A:
B:
An appropriate threshold was defined based on CD45-VioBlue versus side scatter signals, for the exclusion of debris and erythrocytes from the data.
Lymphocytes were gated for their expected morphology using a forward scatter versus side scatter "lymphocyte gate" (P1)
View details

Figure 1

Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4
+
CD25
high
CD127
dim/
cells.
View details

Figure 1

Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4
+
CD25
high
CD127
dim/
cells.
C:
D:
Dead cells were excluded by a CD127-PE versus propidium iodide dot plot (P2).
CD4
+
lymphocytes were gated in P3.
View details

Figure 1

Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4
+
CD25
high
CD127
dim/
cells.
View details

Figure 1

Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4
+
CD25
high
CD127
dim/
cells.
CD25
high
CD127
dim/–
Treg cells were gated by a CD127-PE versus CD25-APC dot plot.
View details

Figure 1

Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4
+
CD25
high
CD127
dim/
cells.

Specifications for Treg Detection Kit (CD4/CD25/CD127), human

Overview

The Treg Detection Kit (CD4/CD25/CD127) has been developed as a simple one-reagent solution for the identification of human regulatory T (Treg) cells by flow cytometry. Applications include
  • Staining and enumeration of CD4+CD25+CD127dim/– regulatory T cells in PBMCs, apheresis products, umbilical cord blood, or whole blood
  • Analysis of Treg cells separated using MACS® Technology

Detailed product information

Background information

The kit combines monoclonal antibodies against the standard Treg cell markers CD4, CD25, and CD127 as well as the leukocyte marker CD45 at optimized titers in one vial. The combination of CD4, CD25, and CD127 staining is the current standard method for the identification of live Treg cells. Several publications have reported a strong correlation between the CD25
+
CD127
dim/–
phenotype, expression of FoxP3, and suppressive function in CD4
+
cells.
1,2

Resources for Treg Detection Kit (CD4/CD25/CD127), human

Documents and Protocols

References for Treg Detection Kit (CD4/CD25/CD127), human

Publications

  1. Seddiki, N. et al. (2006) Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J. Exp. Med. 203: 1693-1700
  2. Liu, W. et al. (2006)
    CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4
    +
    T reg cells.
    J. Exp. Med. 203: 1701-1711

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