The Tissue Stem Cell Pre-Enrichment Kit, mouse was specifically developed for the pre-enrichment of tissue derived stem and progenitor cells from multiple tissue sources. The kit allows the depletion of all generic infiltrating cells such as red- and white blood cells, as well as endothelial cells are efficiently removed by a single step magnetic cell separation procedure.

Data and images for Tissue Stem Cell Pre-Enrichment Kit, mouse

Figures

Figure 1

Mouse mammary gland was dissociated using the Adipose Tissue Dissociation Kit, mouse and rat (# 130-105-808) in combination with the gentleMACS Octo Dissociator. Subsequently, tissue‑resident stem and progenitor cells were pre-enriched using the Tissue Stem Cell Pre-Enrichment Kit, mouse. Cells were fluorescently stained with lineage markers (CD31, CD45, Ter-119 conjugated to APC) and Labeling Check Reagent‑VioBlue and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
Stem cell-enriched fraction
Lineage cell-enriched fraction
View details

Figure 1

Mouse mammary gland was dissociated using the Adipose Tissue Dissociation Kit, mouse and rat (# 130-105-808) in combination with the gentleMACS Octo Dissociator. Subsequently, tissue‑resident stem and progenitor cells were pre-enriched using the Tissue Stem Cell Pre-Enrichment Kit, mouse. Cells were fluorescently stained with lineage markers (CD31, CD45, Ter-119 conjugated to APC) and Labeling Check Reagent‑VioBlue and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Mouse mammary gland was dissociated using the Adipose Tissue Dissociation Kit, mouse and rat (# 130-105-808) in combination with the gentleMACS Octo Dissociator. Subsequently, tissue‑resident stem and progenitor cells were pre-enriched using the Tissue Stem Cell Pre-Enrichment Kit, mouse. Cells were fluorescently stained with lineage markers (CD31, CD45, Ter-119 conjugated to APC) and Labeling Check Reagent‑VioBlue and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Mouse mammary gland was dissociated using the Adipose Tissue Dissociation Kit, mouse and rat (# 130-105-808) in combination with the gentleMACS Octo Dissociator. Subsequently, tissue‑resident stem and progenitor cells were pre-enriched using the Tissue Stem Cell Pre-Enrichment Kit, mouse. Cells were fluorescently stained with lineage markers (CD31, CD45, Ter-119 conjugated to APC) and Labeling Check Reagent‑VioBlue and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Tissue Stem Cell Pre-Enrichment Kit, mouse

Overview

The Tissue Stem Cell Pre-Enrichment Kit, mouse was specifically developed for the pre-enrichment of tissue derived stem and progenitor cells from multiple tissue sources. The kit allows the depletion of all generic infiltrating cells such as red- and white blood cells, as well as endothelial cells are efficiently removed by a single step magnetic cell separation procedure.

Detailed product information

Background information

The Tissue Stem Cell pre-Enrichment Kit, mouse has been designed for the pre-enrichment of untouched tissue resident cell populations, e.g. stem and progenitor cells, from primary specimens. All primary tissues are vascularized and infiltrated by heterogeneous lymphocyte subpopulations, red blood cells, and endothelial cells. The level of heterogeneity is highly dependent on multiple factors like tissue type, age, and disease state. The isolation of target cells from primary tissues, such as organ specific stem and progenitor cells, is frequently hampered by the initial low frequency of these cells. This may lead to long isolation times and impaired purities. By using the Tissue Stem Cell pre-Enrichment Kit, mouse, all generic infiltrating cells, such as red- and white blood cells as well as endothelial cells are efficiently depleted resulting in a significant pre-enrichment of target cells for downstream analysis or sorting.

Columns

LS or autoMACS
®
Columns.

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