Clone:
REA652
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
TCR a/b, IMD7, TCRA, TRAC, TRA, TRB

Extended validation for TCRα/β Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA652
WT31++
T10B9.1A-31++
IP26++
BW242/412++
Cells were incubated with an excess of purified unconjugated TCRα/β (REA652) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for TCRα/β. Human peripheral blood mononuclear cells (PBMCs) were stained with TCRα/β antibodies and with a suitable counterstaining. As a control, TCRα/β antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCRα/β. Human peripheral blood mononuclear cells (PBMCs) were stained with TCRα/β antibodies and with a suitable counterstaining. As a control, TCRα/β antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCRα/β. Human peripheral blood mononuclear cells (PBMCs) were stained with TCRα/β antibodies and with a suitable counterstaining. As a control, TCRα/β antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCRα/β. Human peripheral blood mononuclear cells (PBMCs) were stained with TCRα/β antibodies and with a suitable counterstaining. As a control, TCRα/β antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCRα/β. Human peripheral blood mononuclear cells (PBMCs) were stained with TCRα/β antibodies and with a suitable counterstaining. As a control, TCRα/β antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCRα/β. Human peripheral blood mononuclear cells (PBMCs) were stained with TCRα/β antibodies and with a suitable counterstaining. As a control, TCRα/β antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for TCRα/β. Human peripheral blood mononuclear cells (PBMCs) were stained with TCRα/β antibodies and with a suitable counterstaining. As a control, TCRα/β antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TCRα/β (REA652). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TCRα/β (REA652). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TCRα/β (REA652). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCRα/β Antibody, anti-human, REAfinity™

Overview

Clone REA652 recognizes the human α/β T cell receptor (TCR). The T cell receptor is a heterodimer composed of two transmembrane glycoprotein chains, α and β. In 95% of T cells the TCR consists of an α and β chain, whereas in 5% of T cells it has γ and δ chains. α and β chains are members of the Ig superfamily and consist of a constant and a polymorphic variable region. The variable region of the TCRα/β is involved in recognition of antigenic peptides presented by the MHC complex of antigen-presenting cells.
Additional information: Clone REA652 displays negligible binding to Fc receptors.

Alternative names

TCR a/b, IMD7, TCRA, TRAC, TRA, TRB

Detailed product information

Technical specifications

CloneREA652
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenTCRα/β
Alternative names of antigenTCR a/b, IMD7, TCRA, TRAC, TRA, TRB
Distribution of antigenT cells
Entrez Gene ID6955, 6957
RRIDAB_2733456, AB_2733457, AB_2733168, AB_2733169, AB_2733069, AB_2733070, AB_2726446, AB_2726169, AB_2784479, AB_2784478, AB_2751815, AB_2751759, AB_2733283, AB_2733284, AB_2733693, AB_2733900, AB_2733901, AB_2733692

References for TCRα/β Antibody, anti-human, REAfinity™

Publications

  1. Brenner, M. B. et al. (1986) Identification of a putative second T-cell receptor. Nature 322(6075): 145-149
  2. Call, M. E. et al. (2002) The organizing principle in the formation of the T cell receptor-CD3 complex. Cell 111(7): 967-979
  3. Oettgen, H. C. et al. (1984) Characterization of the two heavy chains of the T3 complex on the surface of human T lymphocytes. J. Biol. Chem. 259(19): 12039-12048

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