Clone:
REA771
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
TCR VD2, TRDV2, hDV102S1

Extended validation for TCR Vδ2 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA771
B6++
IMMU 389-
15D-
123R3++
Cells were incubated with an excess of purified unconjugated TCR Vδ2 (REA771) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vδ2 (REA771). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vδ2 (REA771). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TCR Vδ2 (REA771). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCR Vδ2 Antibody, anti-human, REAfinity™

Overview

Clone REA771 recognizes the Vδ2 TCR chain on human Vγ9Vδ2 T cells, which are the major γ/δ T cell subset in humans. Activated Vδ2 T lymphocytes display strong cytotoxicity against various tumor cells and produce a number of cytokines, including TNF-α and IFN-γ.
Additional information: Clone REA771 displays negligible binding to Fc receptors.

Alternative names

TCR VD2, TRDV2, hDV102S1

Detailed product information

Technical specifications

CloneREA771
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenTCR Vδ2
Alternative names of antigenTCR VD2, TRDV2, hDV102S1
Molecular mass of antigen [kDa]11
Distribution of antigenT cells
Entrez Gene ID28517
RRIDAB_2653970, AB_2653971, AB_2653972, AB_2653973, AB_2653974, AB_2653975, AB_2653976, AB_2653977, AB_2653978, AB_2653979, AB_2653980, AB_2653981, AB_2653982, AB_2653983, AB_2653984, AB_2653985, AB_2653986, AB_2653969

References for TCR Vδ2 Antibody, anti-human, REAfinity™

Publications

  1. Rincon-Orozco, B. et al. (2005) Activation of V gamma 9V delta 2 T cells by NKG2D. J. Immunol. 175(4): 2144-2151
  2. Scotet, E. et al. (2005) Tumor recognition following Vgamma9Vdelta2 T cell receptor interactions with a surface F1-ATPase-related structure and apolipoprotein A-I. Immunity 22(1): 71-80

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