Clone:
REA173
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
TRDV1, hDV101S1

Extended validation for TCR Vδ1 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA173
R9.12++
Cells were incubated with an excess of purified unconjugated TCR Vδ1 (REA173) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for TCR Vδ1. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vδ1 antibodies and with a suitable counterstaining. As a control, TCR Vδ1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Lymphocytes were pregated for the analysis. Cell debris, dead cells, cell doublets, and monocytes were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCR Vδ1. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vδ1 antibodies and with a suitable counterstaining. As a control, TCR Vδ1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Lymphocytes were pregated for the analysis. Cell debris, dead cells, cell doublets, and monocytes were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCR Vδ1. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vδ1 antibodies and with a suitable counterstaining. As a control, TCR Vδ1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Lymphocytes were pregated for the analysis. Cell debris, dead cells, cell doublets, and monocytes were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for TCR Vδ1. Human peripheral blood mononuclear cells (PBMCs) were stained with TCR Vδ1 antibodies and with a suitable counterstaining. As a control, TCR Vδ1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Lymphocytes were pregated for the analysis. Cell debris, dead cells, cell doublets, and monocytes were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vδ1 (REA173). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vδ1 (REA173). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TCR Vδ1 (REA173). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCR Vδ1 Antibody, anti-human, REAfinity™

Overview

Clone REA173 recognizes the Vδ1 chain of γδ T cell receptor (TCR). The TCR δ locus is located within the TCR α locus on chromosome 14 and 6 Vδ segments have been reported so far. γδ T cells comprise a small population of T cells and unlike peripheral circulating αβ T cells, γδ T cells are mainly found in body tissues such as thymus, peripheral lymphoid tissues, intestinal epithelium, and epidermis. Distribution analysis in human oral cavity revealed preferential localisation of Vδ1
+
γδ T cells in intraepithelial region.
Additional information: Clone REA173 displays negligible binding to Fc receptors.

Alternative names

TRDV1, hDV101S1

Detailed product information

Technical specifications

CloneREA173
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenTCR Vδ1
Alternative names of antigenTRDV1, hDV101S1
Distribution of antigenT cells, epithelial cells
Entrez Gene ID28518
RRIDAB_2751495, AB_2733450, AB_2733451, AB_2751427, AB_2751409, AB_2752150, AB_2752099, AB_2784472, AB_2784471, AB_2752151, AB_2752100, AB_2752148, AB_2752097, AB_2784470, AB_2784469, AB_2752149, AB_2752098, AB_2801866, AB_2751531

References for TCR Vδ1 Antibody, anti-human, REAfinity™

Publications

  1. Di Marco Barros, R. et al. (2016) Epithelia use butyrophilin-like molecules to shape organ-specific γδ T cell compartments. Cell 167(1): 203-218
  2. Libero, G. D. et al. (1997) Sentinel function of broadly reactive human gamma delta T cells. Immunol. Today 18(1): 22-26
  3. Pepin, L. F. et al. (1993) Preferential V delta 1 expression among TcR gamma/delta-bearing T cells in human oral epithelium. Scand. J. Immunol. 37(3): 289-294
  4. Siegers, G. et al. (2018) Functional plasticity of gamma delta T cells and breast tumor targets in hypoxia. Front Immunol 9
  5. Hunter, S. et al. (2018) Human liver infiltrating γδ T cells are composed of clonally expanded circulating and tissue-resident populations. J. Hepatol. 69(3)
  6. Zumwalde, N. A. et al. (2016)
    Analysis of immune cells from human mammary ductal epithelial organoids reveals Vδ2
    +
    T cells that efficiently target breast carcinoma cells in the presence of bisphosphonate.
    Cancer Prev Res (Phila) 9: 305-316
  7. Davey, M. S. et al. (2017) Clonal selection in the human Vδ1 T cell repertoire indicates γδ TCR-dependent adaptive immune surveillance. Nat Commun. 8: 14760
  8. Davey, M. et al. (2018) The human Vδ2+ T-cell compartment comprises distinct innate-like Vγ9+ and adaptive Vγ9- subsets. Nat Commun. 9(1)

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