The Switched Memory B Cell Isolation Kit was developed for the fast and efficient isolation of untouched switched memory B cells from human PBMCs.

Data and images for Switched Memory B Cell Isolation Kit, human

Figures

Figure 1

Untouched switched memory B cells were isolated from human PBMCs using the Switched Memory B Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD19-FITC, Anti-IgD-PE, Anti-IgM-PE, Anti-IgA-APC, and Anti-IgG-APC and analyzed by flow cytometry. The cells are gated on CD19
+
cells.
PBMCs before separation
Enriched switched memory B cells
View details

Figure 1

Untouched switched memory B cells were isolated from human PBMCs using the Switched Memory B Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD19-FITC, Anti-IgD-PE, Anti-IgM-PE, Anti-IgA-APC, and Anti-IgG-APC and analyzed by flow cytometry. The cells are gated on CD19
+
cells.
View details

Figure 1

Untouched switched memory B cells were isolated from human PBMCs using the Switched Memory B Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD19-FITC, Anti-IgD-PE, Anti-IgM-PE, Anti-IgA-APC, and Anti-IgG-APC and analyzed by flow cytometry. The cells are gated on CD19
+
cells.
Other B cells
View details

Figure 1

Untouched switched memory B cells were isolated from human PBMCs using the Switched Memory B Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD19-FITC, Anti-IgD-PE, Anti-IgM-PE, Anti-IgA-APC, and Anti-IgG-APC and analyzed by flow cytometry. The cells are gated on CD19
+
cells.

Specifications for Switched Memory B Cell Isolation Kit, human

Overview

The Switched Memory B Cell Isolation Kit was developed for the fast and efficient isolation of untouched switched memory B cells from human PBMCs.

Detailed product information

Background information

Switched Memory B cells are indicators of normal B cell activation and development in germinal centers of lymph nodes or other secondary lymphoid organs. The amount of switched memory B cells in peripheral blood is an indicator for humoral immunodeficiency, such as specific antibody deficiency (SAD) or common variable immunodeficiency (CVID).

Detailed separation procedure

Non-target cells are labeled with a cocktail of biotinylated CD2, CD14, CD16, CD36, CD43, CD235a (glycophorin A), Anti-IgM, and Anti-IgD antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. Highly pure switched memory B cells are obtained by depletion of the magnetically labeled cells.

Downstream applications

Purified switched memory B cells can be used for studies in cell biology or molecular biology and functional assays.

Columns

LS or autoMACS
®
Columns.

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