The StraightFrom
®
MicroBead Kit was developed for the rapid positive selection of CD8
+
cells directly from one LRSC using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS
®
Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. LS Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire LRSC.

Data and images for
StraightFrom
®
LRSC CD8 MicroBead Kit
, human

Figures

Figure 1

Separation of an LRSC sample using the StraightFrom
®
LRSC CD8 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and LS Columns. Cells were fluorescently stained with CD3-APC, CD8-PE, as well as CD45-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
CD8
+
cells
View details

Figure 1

Separation of an LRSC sample using the StraightFrom
®
LRSC CD8 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and LS Columns. Cells were fluorescently stained with CD3-APC, CD8-PE, as well as CD45-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Separation of an LRSC sample using the StraightFrom
®
LRSC CD8 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and LS Columns. Cells were fluorescently stained with CD3-APC, CD8-PE, as well as CD45-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for
StraightFrom
®
LRSC CD8 MicroBead Kit
, human

Overview

The StraightFrom
®
MicroBead Kit was developed for the rapid positive selection of CD8
+
cells directly from one LRSC using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS
®
Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. LS Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire LRSC.

Detailed product information

Background information

In blood the CD8 antigen is expressed on human cytotoxic T cells and on a subset of CD16
+
NK cells. The CD8 antigen acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8
+
cytotoxic T cells.

Detailed separation procedure

One entire LRSC sample is labeled with StraightFrom
®
LRSC CD8 MicroBeads and loaded onto LS Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS
®
Separator. After removing the column from the magnetic field, the magnetically retained CD4
+
T cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD8
+
cells are well suited for further flow cytometric, functional, or molecular analysis.
Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

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StraightFrom
®
LRSC CD8 MicroBead Kit
, human

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