The StraightFrom
®
Buffy Coat CD14 MicroBead Kit has been developed for the rapid positive selection of CD14
+
cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS
®
Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy

Data and images for
StraightFrom
®
Buffy Coat CD14 MicroBead Kit
, human

Figures

Figure 1

Separation of a buffy coat sample using the StraightFrom
®
Buffy Coat CD14 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with CD15-APC, CD14-PE, as well as CD45‑VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Analysis gate
CD14
+
cells
View details

Figure 1

Separation of a buffy coat sample using the StraightFrom
®
Buffy Coat CD14 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with CD15-APC, CD14-PE, as well as CD45‑VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Separation of a buffy coat sample using the StraightFrom
®
Buffy Coat CD14 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with CD15-APC, CD14-PE, as well as CD45‑VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for
StraightFrom
®
Buffy Coat CD14 MicroBead Kit
, human

Overview

The StraightFrom
®
Buffy Coat CD14 MicroBead Kit has been developed for the rapid positive selection of CD14
+
cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS
®
Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy coat.

Detailed product information

Background information

The CD14 antigen belongs to the LPS receptor complex. It is strongly expressed on most monocytes and macrophages and weakly on neutrophils.

Detailed separation procedure

One entire buffy coat is labeled with StraightFrom
®
Buffy Coat CD14 MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS
®
Separator. After removing the column from the magnetic field, the magnetically retained CD14
+
cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD14
+
cells are well-suited for further flow cytometric, functional, or molecular analysis.
Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

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StraightFrom
®
Buffy Coat CD14 MicroBead Kit
, human

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