Clone:
REAL291
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IF, IHC
Alternative names:
SIGLECF, SIGLEC5, mSiglec-F, Siglec-5

Specifications for Siglec-F Antibody, anti-mouse,
REAlease
®

Overview

Clone REAL291 is an antibody fragment derived from the full Siglec-F antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL291 recognizes the mouse siglec-F antigen, also kown as siglec-5, which belongs to the sialic acid-binding immunoglobulin-type lectins (siglec) family. Siglecs are expressed on immune cells and are a subset of the I-type lectins. Siglec-F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. In mouse, siglec-F is an inhibitory receptor on eosinophils.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

SIGLECF, SIGLEC5, mSiglec-F, Siglec-5

Detailed product information

Technical specifications

CloneREAL291
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Speciesmouse
AntigenSiglec-F
Alternative names of antigenSIGLECF, SIGLEC5, mSiglec-F, Siglec-5
Distribution of antigengranulocytes, monocytes, bone marrow
RRIDAB_2751311, AB_2784453, AB_2784452, AB_2751451

References for Siglec-F Antibody, anti-mouse,
REAlease
®

Publications

  1. Tateno, H. et al. (2005) Mouse Siglec-F and human Siglec-8 are functionally convergent paralogs that are selectively expressed on eosinophils and recognize 6-sulfo-sialyl Lewis X as a preferred glycan ligand. Glycobiology 15(11): 1125-1135
  2. Zhang, M. et al. (2007)
    Defining the
    in vivo
    function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils.
    Blood 109(10): 4280-4287
  3. Guo, J. P. et al. (2011) Characterization of expression of glycan ligands for Siglec-F in normal mouse lungs. Am. J. Respir. Cell Mol. Biol. 44(2): 238-243

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Siglec-F Antibody, anti-mouse,
REAlease
®

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