The Satellite Cell Isolation Kit, mouse has been developed for the isolation of vital murine satellite cells from skeletal muscle. Enriched satellite cells are fully functional and can be used for downstream applications, such as differentiation into myotubes.

Data and images for Satellite Cell Isolation Kit, mouse

Figures

Figure 1

Mouse skeletal muscle was dissociated using the Skeletal Muscle Dissociation Kit, mouse and rat (# 130-098-305) in combination with the gentleMACS Dissociator. Subsequently, satellite cells were isolated using the Satellite Cell Isolation Kit, mouse and cultured in expansion medium (40% DMEM, 40% HAM’s F10, 2.5 ng/mL human FGF-2, 100 U/mL penicillin/ 100 U/mL streptomycin) for six days.
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Figure 1

Mouse skeletal muscle was dissociated using the Skeletal Muscle Dissociation Kit, mouse and rat (# 130-098-305) in combination with the gentleMACS Dissociator. Subsequently, satellite cells were isolated using the Satellite Cell Isolation Kit, mouse and cultured in expansion medium (40% DMEM, 40% HAM’s F10, 2.5 ng/mL human FGF-2, 100 U/mL penicillin/ 100 U/mL streptomycin) for six days.
View details

Figure 1

Mouse skeletal muscle was dissociated using the Skeletal Muscle Dissociation Kit, mouse and rat (# 130-098-305) in combination with the gentleMACS Dissociator. Subsequently, satellite cells were isolated using the Satellite Cell Isolation Kit, mouse and cultured in expansion medium (40% DMEM, 40% HAM’s F10, 2.5 ng/mL human FGF-2, 100 U/mL penicillin/ 100 U/mL streptomycin) for six days.

Specifications for Satellite Cell Isolation Kit, mouse

Overview

The Satellite Cell Isolation Kit, mouse has been developed for the isolation of vital murine satellite cells from skeletal muscle. Enriched satellite cells are fully functional and can be used for downstream applications, such as differentiation into myotubes.

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Background information

One of the most used experimental models in tissue regeneration are satellite cells. Especially the isolation and subsequent culture of satellite cells for biochemical, physiological, pharmacological, and morphological studies have a high impact in the field of tissue regeneration. The Satellite Cell Isolation Kit, mouse has been designed for the enrichment of untouched satellite cells from dissociated mouse skeletal muscle. For optimal results, the Satellite Cell Isolation Kit, mouse should be used in combination with the Skeletal Muscle Dissociation Kit, mouse and rat (# 130-098-305).

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LS or autoMACS
®
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