The SARS-CoV-2 T Cell Analysis Kit (Whole Blood), human has been developed for the fast and easy detection of SARS-CoV-2-reactive T cells directly from whole blood samples by intra- and extracellular staining of activation markers and cytokines. The kit contains antibodies for the identification of CD4
+
and CD8
+
T cells, the exclusion of monocytes and B cells as well as the staining of activation markers and cytokines. Furthermore a positive control (CytoStim), Brefeldin A and reagents (Inside Fix and Inside Perm) for the fixation and permeabilization of cells after stimulation are included. The optimized flow panel and protocol ensure a

Data and images for SARS-CoV-2 T Cell Analysis Kit (Whole Blood), human

Figures

Figure 1: Workflow for SARS-CoV-2–reactive T cell analysis in whole blood samples using the kit

View details
Human whole blood samples are incubated with a SARS-CoV-2 PepTivator
®
Peptide Pool of choice and Brefeldin A. Red blood cells are lysed using the Red Blood Cell Lysis Solution. After fixation and permeabilization, cells are stained for lineage and activation markers as well as cytokines using the flow panel provided in the kit. Cells are then analyzed by flow cytometry using, for example, a MACSQuant
®
Analyzer.
Human whole blood samples are incubated with a SARS-CoV-2 PepTivator
®
Peptide Pool of choice and Brefeldin A. Red blood cells are lysed using the Red Blood Cell Lysis Solution. After fixation and permeabilization, cells are stained for lineage and activation markers as well as cytokines using the flow panel provided in the kit. Cells are then analyzed by flow cytometry using, for example, a MACSQuant
®
Analyzer.

Figure 2

Human whole blood (1 mL) from SARS-CoV-2–reactive donors were incubated for 8 hours with Brefeldin A. Simultaneously, cells were either left unstimulated (A) or incubated with a selection of SARS-CoV-2 PepTivators Prot_N (130-126-700) (B) or Prot_S1 (130-127-041) (C). Blood samples were lysed and then fixed and permeabilized afterwards. Subsequently, cells were stained with the flow panel included in this kit. Cells were analyzed using a MACSQuant
®
Analyzer 16. Doublets, debris, and dead cells as well as CD14
+
and CD20
+
cells were excluded. After pregating on CD3 as well as CD4 and CD8, respectively, activation marker and cytokine expression were assessed, e.g. CD154 and IL-2 for CD4
+
T cells and TNF-α and IFN-γ for CD8
+
T cells.
A) Unstimulated cells
View details

Figure 2

Human whole blood (1 mL) from SARS-CoV-2–reactive donors were incubated for 8 hours with Brefeldin A. Simultaneously, cells were either left unstimulated (A) or incubated with a selection of SARS-CoV-2 PepTivators Prot_N (130-126-700) (B) or Prot_S1 (130-127-041) (C). Blood samples were lysed and then fixed and permeabilized afterwards. Subsequently, cells were stained with the flow panel included in this kit. Cells were analyzed using a MACSQuant
®
Analyzer 16. Doublets, debris, and dead cells as well as CD14
+
and CD20
+
cells were excluded. After pregating on CD3 as well as CD4 and CD8, respectively, activation marker and cytokine expression were assessed, e.g. CD154 and IL-2 for CD4
+
T cells and TNF-α and IFN-γ for CD8
+
T cells.
View details

Figure 2

Human whole blood (1 mL) from SARS-CoV-2–reactive donors were incubated for 8 hours with Brefeldin A. Simultaneously, cells were either left unstimulated (A) or incubated with a selection of SARS-CoV-2 PepTivators Prot_N (130-126-700) (B) or Prot_S1 (130-127-041) (C). Blood samples were lysed and then fixed and permeabilized afterwards. Subsequently, cells were stained with the flow panel included in this kit. Cells were analyzed using a MACSQuant
®
Analyzer 16. Doublets, debris, and dead cells as well as CD14
+
and CD20
+
cells were excluded. After pregating on CD3 as well as CD4 and CD8, respectively, activation marker and cytokine expression were assessed, e.g. CD154 and IL-2 for CD4
+
T cells and TNF-α and IFN-γ for CD8
+
T cells.
B) Cells stimulated with PepTivator® SARS-CoV-2 Prot_N
View details

Figure 2

Human whole blood (1 mL) from SARS-CoV-2–reactive donors were incubated for 8 hours with Brefeldin A. Simultaneously, cells were either left unstimulated (A) or incubated with a selection of SARS-CoV-2 PepTivators Prot_N (130-126-700) (B) or Prot_S1 (130-127-041) (C). Blood samples were lysed and then fixed and permeabilized afterwards. Subsequently, cells were stained with the flow panel included in this kit. Cells were analyzed using a MACSQuant
®
Analyzer 16. Doublets, debris, and dead cells as well as CD14
+
and CD20
+
cells were excluded. After pregating on CD3 as well as CD4 and CD8, respectively, activation marker and cytokine expression were assessed, e.g. CD154 and IL-2 for CD4
+
T cells and TNF-α and IFN-γ for CD8
+
T cells.
View details

Figure 2

Human whole blood (1 mL) from SARS-CoV-2–reactive donors were incubated for 8 hours with Brefeldin A. Simultaneously, cells were either left unstimulated (A) or incubated with a selection of SARS-CoV-2 PepTivators Prot_N (130-126-700) (B) or Prot_S1 (130-127-041) (C). Blood samples were lysed and then fixed and permeabilized afterwards. Subsequently, cells were stained with the flow panel included in this kit. Cells were analyzed using a MACSQuant
®
Analyzer 16. Doublets, debris, and dead cells as well as CD14
+
and CD20
+
cells were excluded. After pregating on CD3 as well as CD4 and CD8, respectively, activation marker and cytokine expression were assessed, e.g. CD154 and IL-2 for CD4
+
T cells and TNF-α and IFN-γ for CD8
+
T cells.
C) Cells stimulated with PepTivator® SARS-CoV-2 Prot_S1
View details

Figure 2

Human whole blood (1 mL) from SARS-CoV-2–reactive donors were incubated for 8 hours with Brefeldin A. Simultaneously, cells were either left unstimulated (A) or incubated with a selection of SARS-CoV-2 PepTivators Prot_N (130-126-700) (B) or Prot_S1 (130-127-041) (C). Blood samples were lysed and then fixed and permeabilized afterwards. Subsequently, cells were stained with the flow panel included in this kit. Cells were analyzed using a MACSQuant
®
Analyzer 16. Doublets, debris, and dead cells as well as CD14
+
and CD20
+
cells were excluded. After pregating on CD3 as well as CD4 and CD8, respectively, activation marker and cytokine expression were assessed, e.g. CD154 and IL-2 for CD4
+
T cells and TNF-α and IFN-γ for CD8
+
T cells.
View details

Figure 2

Human whole blood (1 mL) from SARS-CoV-2–reactive donors were incubated for 8 hours with Brefeldin A. Simultaneously, cells were either left unstimulated (A) or incubated with a selection of SARS-CoV-2 PepTivators Prot_N (130-126-700) (B) or Prot_S1 (130-127-041) (C). Blood samples were lysed and then fixed and permeabilized afterwards. Subsequently, cells were stained with the flow panel included in this kit. Cells were analyzed using a MACSQuant
®
Analyzer 16. Doublets, debris, and dead cells as well as CD14
+
and CD20
+
cells were excluded. After pregating on CD3 as well as CD4 and CD8, respectively, activation marker and cytokine expression were assessed, e.g. CD154 and IL-2 for CD4
+
T cells and TNF-α and IFN-γ for CD8
+
T cells.

Specifications for SARS-CoV-2 T Cell Analysis Kit (Whole Blood), human

Overview

The SARS-CoV-2 T Cell Analysis Kit (Whole Blood), human has been developed for the fast and easy detection of SARS-CoV-2-reactive T cells directly from whole blood samples by intra- and extracellular staining of activation markers and cytokines. The kit contains antibodies for the identification of CD4
+
and CD8
+
T cells, the exclusion of monocytes and B cells as well as the staining of activation markers and cytokines. Furthermore a positive control (CytoStim), Brefeldin A and reagents (Inside Fix and Inside Perm) for the fixation and permeabilization of cells after stimulation are included. The optimized flow panel and protocol ensure a comprehensive and efficient analysis of SARS-CoV-2-reactive T cells in whole blood. SARS-CoV-2 PepTivator
®
Peptide Pool of choice can be combined according to the application needs.
Note: PepTivator Peptide Pools are not included and have to be purchased separately. Please note that you are purchasing the appropriate quantity of PepTivator Peptide Pools.

Detailed product information

Background information

T lymphocytes execute and control immunological reactions with a repertoire of cytokines, cytotoxic substances, and other mediators. The quantitative and qualitative analysis of CD4
+
T cells and CD8+ T cells specifically recognizing and reacting towards a defined antigen provide important information to understand their function in various immunological situations. In the context of COVID-19, SARS-CoV-2–reactive T cells are induced. The presence of these cells indicates an infected or convalescent donor and may also allow conclusions on disease progression, severity, specific immune reaction and status. Antigen-reactive T cells can thereby be identified and characterized by analyzing their effector function, e.g., upregulation of activation markers and production of cytokines.

Resources for SARS-CoV-2 T Cell Analysis Kit (Whole Blood), human

Documents and Protocols

Brochures/posters

COVID-19 research flyer

References for SARS-CoV-2 T Cell Analysis Kit (Whole Blood), human

Publications

  1. Bacher, P. et al. (2020)
    Low avidity CD4
    +
    T cell responses to SARS-CoV-2 in unexposed individuals and humans with severe COVID-19
    Immunity 53: 1258-1271
  2. Braun, J. et al. (2020) SARS-CoV-2-reactive T cells in healthy donors and patients with COVID-19 Nature 587(7833): 270-274
  3. Meckiff, J. et al. (2020)
    Imbalance of Regulatory and Cytotoxic SARS-CoV-2-Reactive CD4
    +
    T Cells in COVID-19
    Cell 183(5): 1340-1353
  4. Swadling, L. et al. (2020) Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence. Cell Rep 28(4): 1063-1073
  5. Kusnadi, A. et al. (2021)
    Severely ill COVID-19 patients display augmented functional properties in SARS-CoV-2-reactive CD8
    +
    T cells
    Sci Immunol 6: eabe4782
  6. Thieme, C. J. et al. (2020) Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients. Cell Rep Med. 1(6): 100092

Related products for
SARS-CoV-2 T Cell Analysis Kit (Whole Blood), human

16 products available | view all

Seems like you are coming from USA!
Do you want to visit our website in your country?