The SARS-CoV-2 S2 protein (Spike glycoprotein subunit 2) is localized at the C-terminus of the Spike protein and mediates membrane penetration and virus–cell fusion through the presence of so called heptad repeats. The SARS-CoV-2 Spike-S2 (insect cells) protein covers amino acids S686 to K1211 and contains the stabilizing proline substitutions at position K986P and V987P. The protein is extended at its C-terminus with a His-tag and an AviTag
TM
.

Data and images for Recombinant SARS-CoV-2 Spike-S2 (insect cells)

Figures

Figure 1

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Bead-based immunoassay for detection of patient-derived SARS-CoV-2 antibodies
. Schematic overview illustrating how our biotinylated antigens can be applied to detect patient-derived SARS-CoV-2 antibodies

Figure 1

Bead-based immunoassay for detection of patient-derived SARS-CoV-2 antibodies
. Schematic overview illustrating how our biotinylated antigens can be applied to detect patient-derived SARS-CoV-2 antibodies

Figure 2

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Results of the bead-based immunoassay using biotinylated
Recombinant SARS-CoV-2 Spike-S2 (insect cells). The amount of captured SARS-CoV-2 antibodies was quantified at different plasma dilutions. Results for plasma from a COVID-19
+
patient (circle) or a healthy donor (square) are shown.

Figure 2

Results of the bead-based immunoassay using biotinylated
Recombinant SARS-CoV-2 Spike-S2 (insect cells). The amount of captured SARS-CoV-2 antibodies was quantified at different plasma dilutions. Results for plasma from a COVID-19
+
patient (circle) or a healthy donor (square) are shown.

Figure 3

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ELISA of Recombinant SARS-CoV-2 Spike-S2 (insect cells).
Recombinant SARS-CoV-2 Spike-S2 (insect cells)-Biotin can be detected by ELISA assays. Quantification at different protein concentrations illustrates that Miltenyi Biotec's recombinant S2 protein is recognized by anti-S2 antibodies.

Figure 3

ELISA of Recombinant SARS-CoV-2 Spike-S2 (insect cells).
Recombinant SARS-CoV-2 Spike-S2 (insect cells)-Biotin can be detected by ELISA assays. Quantification at different protein concentrations illustrates that Miltenyi Biotec's recombinant S2 protein is recognized by anti-S2 antibodies.

Figure 4

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SDS-PAGE of biotinylated Recombinant SARS-CoV-2 Spike-S2
(insect cells) under reduced (R) and non-reduced (NR) conditions.

Figure 4

SDS-PAGE of biotinylated Recombinant SARS-CoV-2 Spike-S2
(insect cells) under reduced (R) and non-reduced (NR) conditions.

Figure 5

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Mass spectrometry analysis (ESI-MS) of Recombinant SARS-CoV-2 Spike-S2 (insect cells).
The non-biotinylated protein was analyzed and the peak corresponds to the deglycosylated molecular mass (61233 Da).

Figure 5

Mass spectrometry analysis (ESI-MS) of Recombinant SARS-CoV-2 Spike-S2 (insect cells).
The non-biotinylated protein was analyzed and the peak corresponds to the deglycosylated molecular mass (61233 Da).

Specifications for Recombinant SARS-CoV-2 Spike-S2 (insect cells)

Overview

The SARS-CoV-2 S2 protein (Spike glycoprotein subunit 2) is localized at the C-terminus of the Spike protein and mediates membrane penetration and virus–cell fusion through the presence of so called heptad repeats. The SARS-CoV-2 Spike-S2 (insect cells) protein covers amino acids S686 to K1211 and contains the stabilizing proline substitutions at position K986P and V987P. The protein is extended at its C-terminus with a His-tag and an AviTag
TM
.

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