The Recombinant SARS-CoV-2 Nucleoprotein (HEK) (also known as nucleocapsid protein or protein N) is highly immunogenic and represents a crucial structural molecule of the SARS-CoV-2 virus. The N-terminal RNA-binding domain (NTD) associates with the viral genome of SARS-CoV-2. An intrinsically disordered stretch connects the NTD domain with the C-terminal dimerization domain (CTD). A major function of the nucleocapsid protein is the packaging of the viral genome into helical nucleocapsid structures. The recombinant SARS-CoV-2 nucleoprotein construct covers the full length protein (amino acids 2S–419A) and contains a His-tag

Data and images for Recombinant SARS-CoV-2 Nucleoprotein

Figures

Figure 1

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Bead-based immunoassay for detection of patient-derived SARS-CoV-2 antibodies
. Schematic overview illustrating how our biotinylated antigens can be applied to detect patient-derived SARS-CoV-2 antibodies

Figure 1

Bead-based immunoassay for detection of patient-derived SARS-CoV-2 antibodies
. Schematic overview illustrating how our biotinylated antigens can be applied to detect patient-derived SARS-CoV-2 antibodies

Figure 2

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Results of the bead-based immunoassay using biotinylated Recombinant SARS-CoV-2 Nucleoprotein.
The amount of captured SARS-CoV-2 antibodies was quantified at different plasma dilutions. Results for plasma from a COVID-19
+
patient (circle) or a healthy donor (square) are shown.

Figure 2

Results of the bead-based immunoassay using biotinylated Recombinant SARS-CoV-2 Nucleoprotein.
The amount of captured SARS-CoV-2 antibodies was quantified at different plasma dilutions. Results for plasma from a COVID-19
+
patient (circle) or a healthy donor (square) are shown.

Figure 3

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ELISA of Recombinant SARS-CoV-2 Nucleoprotein.
Binding of biotinylated Recombinant SARS-CoV-2 Nucleoprotein to a N-protein-secific antibody was detected.

Figure 3

ELISA of Recombinant SARS-CoV-2 Nucleoprotein.
Binding of biotinylated Recombinant SARS-CoV-2 Nucleoprotein to a N-protein-secific antibody was detected.

Figure 4

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SDS-PAGE of non-biotinylated Recombinant SARS-CoV-2 Nucleoprotein
under reduced (R) and non-reduced (NR) conditions.

Figure 4

SDS-PAGE of non-biotinylated Recombinant SARS-CoV-2 Nucleoprotein
under reduced (R) and non-reduced (NR) conditions.

Figure 5

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Mass spectrometry analysis (ESI-MS) of Recombinant SARS-CoV-2 Nucleoprotein.
The non-biotinylated protein was analyzed and the peak corresponds to the O-glycosylated molecular mass (50056 Da).

Figure 5

Mass spectrometry analysis (ESI-MS) of Recombinant SARS-CoV-2 Nucleoprotein.
The non-biotinylated protein was analyzed and the peak corresponds to the O-glycosylated molecular mass (50056 Da).

Specifications for Recombinant SARS-CoV-2 Nucleoprotein

Overview

The Recombinant SARS-CoV-2 Nucleoprotein (HEK) (also known as nucleocapsid protein or protein N) is highly immunogenic and represents a crucial structural molecule of the SARS-CoV-2 virus. The N-terminal RNA-binding domain (NTD) associates with the viral genome of SARS-CoV-2. An intrinsically disordered stretch connects the NTD domain with the C-terminal dimerization domain (CTD). A major function of the nucleocapsid protein is the packaging of the viral genome into helical nucleocapsid structures. The recombinant SARS-CoV-2 nucleoprotein construct covers the full length protein (amino acids 2S–419A) and contains a His-tag and an AviTag™. Recombinant SARS-CoV-2 Nucleoprotein (HEK)-Biotin is specifically biotinylated at a single site, preserving the full functionality of the protein.

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Recombinant SARS-CoV-2 Nucleoprotein

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