The REAlease
®
CD56 MicroBead Kit has been developed for positive selection CD56
+
cells from PBMCs. The kit contains an indirect magnetic labeling system for obtaining target cells free of MicroBeads and REAlease Biotin Complex.

Data and images for
REAlease
®
CD56 MicroBead Kit
, human

Figures

Figure 1

View details
Step-by-step REAlease MicroBeads separation procedure

Figure 1

Step-by-step REAlease MicroBeads separation procedure

Figure 2

Example of a separation using the REAlease® CD56 MicroBead Kit
A) CD56
+
cells were isolated from human PBMCs using the REAlease
®
CD56 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD56-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD56
+
cells"), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD56).
A) Cell purity
Before separation
Label-free CD56
+
cells
View details

Figure 2

Example of a separation using the REAlease® CD56 MicroBead Kit
A) CD56
+
cells were isolated from human PBMCs using the REAlease
®
CD56 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD56-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD56
+
cells"), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD56).
View details

Figure 2

Example of a separation using the REAlease® CD56 MicroBead Kit
A) CD56
+
cells were isolated from human PBMCs using the REAlease
®
CD56 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD56-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD56
+
cells"), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD56).
B) Label-free cells: REAlease Biotin Complex release
Before separation
MicroBead-free CD56
+
cells
Label-free CD56
+
cells
View details

Figure 2

Example of a separation using the REAlease® CD56 MicroBead Kit
A) CD56
+
cells were isolated from human PBMCs using the REAlease
®
CD56 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD56-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD56
+
cells"), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD56).
View details

Figure 2

Example of a separation using the REAlease® CD56 MicroBead Kit
A) CD56
+
cells were isolated from human PBMCs using the REAlease
®
CD56 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD56-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD56
+
cells"), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD56).
View details

Figure 2

Example of a separation using the REAlease® CD56 MicroBead Kit
A) CD56
+
cells were isolated from human PBMCs using the REAlease
®
CD56 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD56-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD56
+
cells"), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD56).

Specifications for
REAlease
®
CD56 MicroBead Kit
, human

Overview

The REAlease
®
CD56 MicroBead Kit has been developed for positive selection CD56
+
cells from PBMCs. The kit contains an indirect magnetic labeling system for obtaining target cells free of MicroBeads and REAlease Biotin Complex.

Detailed product information

Background information

The CD56 antigen is expressed by most NK cells and a T cell subset. Upon activation of NK cells, the surface expression of CD56 is increased.

Downstream applications

  • Isolation of specific CD56+ cell subsets. The CD56+ cells can be used to isolate specific NK or T cell subsets, e.g., CD8+ CD56+ , CD3+ CD56+ , CD3- CD56+, or CD56+ CD69+ cell subsets.
  • Isolation of CD56+ cell subset which needs to be label-free.

Resources for
REAlease
®
CD56 MicroBead Kit
, human

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®
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, human

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