The REAlease
®
CD4 MicroBead Kit has been developed for positive selection of CD4
+
T cells from PBMCs. The kit contains an indirect magnetic labeling system for obtaining target cells free of MicroBeads and REAlease Biotin Complex.

Data and images for
REAlease
®
CD4 MicroBead Kit
, human

Figures

Figure 1

View details
Step-by-step REAlease MicroBeads separation procedure

Figure 1

Step-by-step REAlease MicroBeads separation procedure

Figure 2

Example of a separation using the REAlease® CD4 MicroBead Kit
A) CD4
+
T cells were isolated from human PBMCs using the REAlease
®
CD4 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-FITC and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4
+
cells"), whereas the label-free CD4
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4).
A) Cell purity
Before separation
Label-free CD4
+
cells
View details

Figure 2

Example of a separation using the REAlease® CD4 MicroBead Kit
A) CD4
+
T cells were isolated from human PBMCs using the REAlease
®
CD4 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-FITC and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4
+
cells"), whereas the label-free CD4
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4).
View details

Figure 2

Example of a separation using the REAlease® CD4 MicroBead Kit
A) CD4
+
T cells were isolated from human PBMCs using the REAlease
®
CD4 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-FITC and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4
+
cells"), whereas the label-free CD4
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4).
B) Label-free cells: REAlease Biotin Complex release
Before separation
MicroBead-free CD4
+
cells
Label-free CD4
+
cells
View details

Figure 2

Example of a separation using the REAlease® CD4 MicroBead Kit
A) CD4
+
T cells were isolated from human PBMCs using the REAlease
®
CD4 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-FITC and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4
+
cells"), whereas the label-free CD4
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4).
View details

Figure 2

Example of a separation using the REAlease® CD4 MicroBead Kit
A) CD4
+
T cells were isolated from human PBMCs using the REAlease
®
CD4 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-FITC and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4
+
cells"), whereas the label-free CD4
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4).
View details

Figure 2

Example of a separation using the REAlease® CD4 MicroBead Kit
A) CD4
+
T cells were isolated from human PBMCs using the REAlease
®
CD4 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-FITC and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD4
+
cells"), whereas the label-free CD4
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD4).

Specifications for
REAlease
®
CD4 MicroBead Kit
, human

Overview

The REAlease
®
CD4 MicroBead Kit has been developed for positive selection of CD4
+
T cells from PBMCs. The kit contains an indirect magnetic labeling system for obtaining target cells free of MicroBeads and REAlease Biotin Complex.

Detailed product information

Background information

CD4 is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimers on CD4
+
T helper cells. CD4 is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 molecule is the receptor for the human immunodeficiency virus. The CD4 antibody recognizes most thymocytes and about 65% of all peripheral blood T cells.

Downstream applications

  • Isolation of specific CD4+ T cell subsets. The selected CD4+ T helper cell population can be sorted according to a second marker of interest, for example, CD45RA, CD45RO, CD25, CD27, CD28, CD30, CD62L, or CD69.
  • Isolation of CD4+ T cells which need to be label-free.

Resources for
REAlease
®
CD4 MicroBead Kit
, human

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®
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