Clone:
REA433
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS, IF, IHC
Alternative names:
RB1, OSRC, PPP1R130, RB, p105-Rb, pRb, pp110

Specifications for Rb pS780 Antibody, anti-human, REAfinity™

Overview

Clone REA433 recognizes the human retinoblastoma (Rb) antigen phosphorylated at serine 780 (pS780). The Rb protein is a tumor suppressor that plays a pivotal role in the negative control of the cell cycle and in tumor progression. It has been shown that Rb is responsible for a major G1 checkpoint, blocking S-phase entry, and cell growth. Loss of Rb functions may induce cell cycle deregulation, leading to a malignant phenotype. Rb negatively regulates transcription by forming complexes with members of the E2F transcription factor family. These complexes, when bound to E2F sites within certain target genes, actively repress transcription through a variety of mechanisms including physical interaction with adjacent transcriptional activation domains and recruitment of proteins that directly, or indirectly, lead to histone deacetylation. Rb function is, in turn, modulated by phosphorylation mediated by cyclin-dependent kinases. Phospho-Rb is described as being hyperphosphorylated and when in this state, it is unable to complex E2F and therefore, unable to restrict progression from the G1 phase to the S phase of the cell cycle. During the M-to-G1 transition, phospho-Rb is progressively dephosphorylated by PP1, returning to its growth-suppressive hypophosphorylated state. The pS780-Rb epitope is a robust marker of mitosis that allows cytometric detection of mitotic cells beginning with chromatin condensation and ending after cytokinesis.
Additional information: Clone REA433 displays negligible binding to Fc receptors.

Alternative names

RB1, OSRC, PPP1R130, RB, p105-Rb, pRb, pp110

Detailed product information

Technical specifications

CloneREA433
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenRb pS780
Alternative names of antigenRB1, OSRC, PPP1R130, RB, p105-Rb, pRb, pp110
Molecular mass of antigen [kDa]106
Entrez Gene ID5925
RRIDAB_2653349, AB_2653350, AB_2653351, AB_2653352

References for Rb pS780 Antibody, anti-human, REAfinity™

Publications

  1. Lee, W. H. et al. (1987) The retinoblastoma susceptibility gene encodes a nuclear phosphoprotein associated with DNA binding activity. Nature 329(6140): 642-645
  2. Nair, J. S. et al. (2009) Aurora B kinase regulates the postmitotic endoreduplication checkpoint via phosphorylation of the retinoblastoma protein at serine 780. Mol. Biol. Cell 20(8): 2218-2228
  3. Rubin, S. M. (2013) Deciphering the retinoblastoma protein phosphorylation code. Trends Biochem. Sci. 38(1): 12-19

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