Name: PepTivator® SARS-CoV-2 Select
Availability: InStock

Specifications
References

Specifications for
PepTivator
^®
SARS-CoV-2 Select

Overview

PepTivator

^®

SARS-CoV-2 Select – premium grade is a peptide pool of 88 lyophilized oligopeptides, consisting of MHC class I and class II-restricted peptides derived from the whole proteome of SARS-CoV-2.

In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Applications

The in vitro stimulation of SARS-CoV-2–specific CD4

⁺

and CD8

⁺

T cells with PepTivator

^®

SARS-CoV-2 Select causes secretion of effector cytokines and upregulation of activation markers, which then allow the detection and isolation of SARS-CoV-2–specific T cells:

Detection and analysis of antigen-specific CD4

⁺

and CD8

⁺

effector/memory T cells in PBMCs by MACS

^®

Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.

Isolation of viable antigen-specific CD4

⁺

T cells with the CD154 MicroBead Kit, or of viable CD4

⁺

and CD8

⁺

T cells using MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.

Generation antigen-specific CD4

⁺

and CD8

⁺

effector/ memory T cells from naive T cell populations.

Pulsing of antigen-presenting cells e.g. for research on dendritic cell vaccination.

Detailed product information

Background information

SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), also known as novel coronavirus 2019-nCoV, causes fever, severe respiratory illness and can lead to life threatening pneumonia. The first cases of this disease, termed COVID-19 for coronavirus disease 2019, have been detected in December 2019 in Wuhan, China.

The PepTivator

^®

SARS-CoV-2 Select pool contains 88 peptides of 9 – 22 aa in length. 63 of these peptides are MHC class I-restricted and 25 peptides are MHC class II-restricted. The peptides originate from structural proteins (S, M, N, E) as well as non-structural proteins.

The peptides are restricted to the following HLA-molecules: HLA-A*01, A*02, A*03, A*11, A*24, A*29, A*30, A*31, A*68, B*07, B*08, B*15, B*27, B*35, B*40, B*44, B*54, B*58, C*07, DRB1*01, DRB1*03, DRB1*04, DRB1*07, DRB1*11, DRB1*15.

References for
PepTivator
^®
SARS-CoV-2 Select

Publications

Anft, M. et al. (2020) COVID-19 progression is potentially driven by T cell immunopathogenesis medRxiv - the preprint server for health sciences (doi.org/10.1101/2020.04.28.20083089 )
Thieme, C. J. et al. (2020) The SARS-CoV-2 T-cell immunity is directed against the spike, membrane, and nucleocapsid protein and associated with COVID 19 severity medRxiv - the preprint server for health sciences (doi.org/10.1101/2020.05.13.20100636 )

Specifications

Specifications for
PepTivator
^®
SARS-CoV-2 Select

Overview

PepTivator

^®

SARS-CoV-2 Select – premium grade is a peptide pool of 88 lyophilized oligopeptides, consisting of MHC class I and class II-restricted peptides derived from the whole proteome of SARS-CoV-2.

Applications

The in vitro stimulation of SARS-CoV-2–specific CD4

⁺

and CD8

⁺

T cells with PepTivator

^®

SARS-CoV-2 Select causes secretion of effector cytokines and upregulation of activation markers, which then allow the detection and isolation of SARS-CoV-2–specific T cells:

Detection and analysis of antigen-specific CD4

⁺

and CD8

⁺

effector/memory T cells in PBMCs by MACS

^®

Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.

Isolation of viable antigen-specific CD4

⁺

T cells with the CD154 MicroBead Kit, or of viable CD4

⁺

and CD8

⁺

T cells using MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.

Generation antigen-specific CD4

⁺

and CD8

⁺

effector/ memory T cells from naive T cell populations.

Pulsing of antigen-presenting cells e.g. for research on dendritic cell vaccination.

Detailed product information

Background information

The PepTivator

^®

References

References for
PepTivator
^®
SARS-CoV-2 Select

Publications

Anft, M. et al. (2020) COVID-19 progression is potentially driven by T cell immunopathogenesis medRxiv - the preprint server for health sciences (doi.org/10.1101/2020.04.28.20083089 )
Thieme, C. J. et al. (2020) The SARS-CoV-2 T-cell immunity is directed against the spike, membrane, and nucleocapsid protein and associated with COVID 19 severity medRxiv - the preprint server for health sciences (doi.org/10.1101/2020.05.13.20100636 )

PepTivator
^®
SARS-CoV-2 Select

PepTivator
^®
SARS-CoV-2 Select

Product overview

Specifications for
PepTivator
^®
SARS-CoV-2 Select

Overview

Applications

Detailed product information

Background information

References for
PepTivator
^®
SARS-CoV-2 Select

Publications

Specifications

Specifications for
PepTivator
^®
SARS-CoV-2 Select

Overview

Applications

Detailed product information

Background information

References

References for
PepTivator
^®
SARS-CoV-2 Select

Publications

Related products for

PepTivator
^®
SARS-CoV-2 Select

4 products available

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About Miltenyi Biotec

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PepTivator® SARS-CoV-2 Select

PepTivator® SARS-CoV-2 Select

Product overview

Specifications for PepTivator® SARS-CoV-2 Select

Overview

Applications

Detailed product information

Background information

References for PepTivator® SARS-CoV-2 Select

Publications

Specifications

Specifications for PepTivator® SARS-CoV-2 Select

Overview

Applications

Detailed product information

Background information

References

References for PepTivator® SARS-CoV-2 Select

Publications

Related products for PepTivator® SARS-CoV-2 Select

4 products available

PepTivator® SARS-CoV-2 Prot_S

130-054-201

IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE), human

130-097-343

Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit, human

170-076-017

MACS GMP PepTivator SARS-CoV-2 Select

Shop

Careers

About Miltenyi Biotec

PepTivator
^®
SARS-CoV-2 Select

PepTivator
^®
SARS-CoV-2 Select

Specifications for
PepTivator
^®
SARS-CoV-2 Select

References for
PepTivator
^®
SARS-CoV-2 Select

Specifications for
PepTivator
^®
SARS-CoV-2 Select

References for
PepTivator
^®
SARS-CoV-2 Select

Related products for

PepTivator
^®
SARS-CoV-2 Select

PepTivator
^®
SARS-CoV-2 Prot_S