Alternative names:
SARS-CoV-2 Gamma variant

Specifications for
PepTivator
®
SARS-CoV-2 Prot_S P.1

Overview

PepTivators are pools of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap. The PepTivator SARS-CoV-2 Prot_S P.1 Mutation Pool covers selectively the mutated regions in the surface or spike glycoprotein (“S”) of the SARS-CoV-2 P.1 lineage (Gamma variant). PepTivator SARS-CoV-2 Prot_S P.1 WT Reference Pool consists of the 41 homologous peptides of the Wuhan sequence and serves as a control.
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Alternative names

SARS-CoV-2 Gamma variant

Detailed product information

Background information

SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) was first detected in December 2019 in Wuhan, China. Since that time the virus kept mutating, leading to new virus variants. The P.1 lineage, also known as Gamma variant, 20J/501Y.V3 or VOC-202101/02, is a SARS-CoV-2 variant that originated in Brazil’s Amazonas state. The mutations of this variant affect also immune-relevant parts of structural SARS-CoV-2 proteins and the respective sequences are available in GISAID, a genome data base, e.g. sequence EPI_ ISL_792682. The P.1 lineage (Gamma variant) has been found to evade a pre-existing antibody response and has become the dominant strain in the region of Manaus, Brazil.
In total there are 12 mutations in the surface or spike glycoprotein (“S”) of the P.1 lineage (Gamma variant) compared to the Wuhan variant (reference genome GenBank MN908947.3): L18F, T20N, P26S, D138Y, R190S
K417T, E484K, N501Y, D614G, H655Y, T1027I and V1176F.
The PepTivator SARS-CoV-2 Prot_S P.1 Mutation Pool covers selectively the mutated regions. It consists of 41 peptides of 15 aa length. The PepTivator SARS-CoV-2 Prot_S P.1 Mutation Pool can be used to supplement PepTivators covering the sequence of the wild-type spike glycoprotein (“S”) in order to detect immune responses towards both variants, Wuhan (wild type) variant and P.1 lineage (Gamma variant). PepTivator Peptide pools spanning the sequence of the spike glycoprotein (“S”) of the Wuhan (wild-type) variant are PepTivator SARS-CoV-2 Prot_S (predicted immunodominant sequence domains), Prot_S1 (N-terminal S1 domain), Prot_S+ (part of the C-terminal S2 domain) and Prot_S Complete (all functional domains).
The PepTivator SARS-CoV-2 Prot_S P.1 WT Reference Pool consists of the 41 homologous peptides of the Wuhan sequence and covers L18, T20, P26, D138, R190, K417, E484, N501, D614, H655, T1027
and V1176 of the Wuhan variant (reference genome GenBank MN908947.3). PepTivator SARS-CoV-2 Prot_S P.1 WT Reference Pool serves as a control for the SARS-CoV-2 Prot_S P.1 Mutation Pool. Comparison of the T cell immune response upon stimulation with mutation and reference pool can be used to analyze mutationspecific T cell responses.
 

Applications

The in vitro stimulation of SARS-CoV-2–specific CD4
+
and CD8
+
T cells with PepTivators causes secretion of effector cytokines and upregulation of activation markers, which then allow the detection and isolation of SARS-CoV-2-specific T cells:
  • Detection and analysis of antigen-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable antigen-specific CD4+ T cells with the CD154 MicroBead Kit, or of viable CD4+ and CD8+ T cells using MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation antigen-specific CD4+ and CD8+ effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells e.g. for research on dendritic cell vaccination.

Resources for
PepTivator
®
SARS-CoV-2 Prot_S P.1

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