The PepTivator
®
CMV IE-1 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid overlap, covering the complete sequence of the cytomegalovirus IE-1 protein (UniProt ID: P13202).
PepTivator peptide pools have been especially developed for the efficient
in vitro
stimulation of antigen–specific CD4
+
and CD8
+
T cells, as peptides of 15 aa length with 11-aa overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
1
Quantitative,

Specifications for
PepTivator
®
CMV IE-1
, human

Overview

The PepTivator
®
CMV IE-1 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid overlap, covering the complete sequence of the cytomegalovirus IE-1 protein (UniProt ID: P13202).
PepTivator peptide pools have been especially developed for the efficient
in vitro
stimulation of antigen–specific CD4
+
and CD8
+
T cells, as peptides of 15 aa length with 11-aa overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
1
Quantitative, phenotypical, or functional analysis of CMV IE-1–specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals.

Detailed product information

Background information

CMV IE-1 (immediate early protein 1), also known as UL123, is a non-structural protein, one of the first CMV antigens expressed in an infected cell, and predominantly induces a CD8
+
T cell response. IE-1-specific T cells occur in infected individuals at frequencies comparable to those of pp65-specific CD8
+
T cells.
2
Both CMV antigens, IE-1 and pp65, are considered dominant T cell targets.

Downstream applications

The
in vitro
stimulation of IE-1-specific CD4
+
and CD8
+
T cells with PepTivator CMV IE-1 causes the secretion of effector cytokines and the upregulation of activation markers, which then allow the detection and isolation of IE-1-specific T cells
3
.
  • Detection and analysis of CMV IE-1-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular staining, or other technologies.
  • Isolation of viable CMV IE-1-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay - Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of CMV IE-1-specific CD4+ and CD8+ effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells for research on dendritic cell vaccination.

References for
PepTivator
®
CMV IE-1
, human

Publications

  1. Kiecker, F. et al. (2004) Analysis of antigen-specific T-cell responses with synthetic peptides--what kind of peptide for which purpose? Hum. Immunol. 65: 523-536
  2. Kern, F. et al. (1999)
    Target structures of the CD8
    +
    -T-cell response to human cytomegalovirus: the 72-kilodalton major immediate-early protein revisited.
    J. Virol. 73: 8179-8184
  3. Zandvliet, M. et al. (2010) Co-ordinated isolation of CD8(+) and CD4(+) T cells recognizing a broad repertoire of cytomegalovirus pp65 and IE1 epitopes for highly specific adoptive immunotherapy. Cytotherapy 12: 933-944

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PepTivator
®
CMV IE-1
, human

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