PepTivator
®
BKV VP1 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human BK virus (BKV) VP1 capsid protein (UniProt ID: P14996).
The
in vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Data and images for
PepTivator
®
BKV VP1
, human

Figures

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
A:
Unstimulated control
Stimulated sample
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
B:
Unstimulated control
Stimulated sample
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
C:
Unstimulated control
Stimulated sample
Positive control
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells by intracellular staining using MACS® Rapid Cytokine Inspector (CD4/CD8 T Cell) Kit (# 130-097-343)
From a BKV
+
donor, 10
6
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). PBMCs stimulated with PepTivator CEF MHC Class I Plus (# 130-098-426) were used as positive control.
The cells were stained with the CD4/CD8 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE as described and were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris, monocytes, and B cells were excluded from the analysis. IFN-γ expression was analyzed for CD4
+
and CD8
+
T cells.

Specifications for
PepTivator
®
BKV VP1
, human

Overview

PepTivator
®
BKV VP1 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human BK virus (BKV) VP1 capsid protein (UniProt ID: P14996).
The
in vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Detailed product information

Background information

BKV is a ubiquitous polyomavirus. After primary infection BKV persists in a latent state. Virus reactivation might occur in immunodeficiency or during immunosuppression. VP1 is the major capsid protein of BKV, expressed late in the lytic cycle.

Downstream applications

PepTivator BKV VP1 – research grade has been specifically developed for efficient
in vitro
stimulation of BKV VP1–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications, including:
  • Detection and analysis of BKV VP1–specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable BKV VP1–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of BKV VP1–specific effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells.

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PepTivator
®
BKV VP1
, human

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