PepTivator
®
BKV LT is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human BK virus (BKV) large T (LT) antigen (UniProt ID: P14999).
The
in vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Data and images for
PepTivator
®
BKV LT
, human

Figures

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
A:
Unstimulated
Before enrichment
After enrichment
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
B:
Unstimulated
Before enrichment
After enrichment
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
C:
Unstimulated
Before enrichment
After enrichment
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
View details

Figure 1

Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV
+
donor, 10
7
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator
®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.

Specifications for
PepTivator
®
BKV LT
, human

Overview

PepTivator
®
BKV LT is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human BK virus (BKV) large T (LT) antigen (UniProt ID: P14999).
The
in vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Detailed product information

Background information

BKV is a ubiquitous polyomavirus. After primary infection BKV persists in a latent state. Virus reactivation might occur in immunodeficiency or during immunosuppression. The LT antigen is expressed early in the lytic cycle and is involved in virus replication.

Downstream applications

PepTivator BKV LT - research grade has been specifically developed for efficient
in vitro
stimulation of BKV LT–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications, including:
  • Detection and analysis of BKV LT–specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable BKV LT–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of BKV LT–specific effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells.

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PepTivator
®
BKV LT
, human

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