The Pan B Cell Isolation Kit II was developed for the fast and efficient isolation of untouched murine B-1 and B-2 B cell subsets from single-cell suspensions of lymphoid tissues.

Data and images for Pan B Cell Isolation Kit II, mouse

Figures

Figure 1

Pan B cells were isolated from spleen of a BALB/c mouse by using the Pan B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD19-APC (# 130-102-494) and CD45-FITC (# 130-102-491) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
After separation
View details

Figure 1

Pan B cells were isolated from spleen of a BALB/c mouse by using the Pan B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD19-APC (# 130-102-494) and CD45-FITC (# 130-102-491) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Pan B cells were isolated from spleen of a BALB/c mouse by using the Pan B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD19-APC (# 130-102-494) and CD45-FITC (# 130-102-491) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Pan B Cell Isolation Kit II, mouse

Overview

The Pan B Cell Isolation Kit II was developed for the fast and efficient isolation of untouched murine B-1 and B-2 B cell subsets from single-cell suspensions of lymphoid tissues.

Detailed product information

Detailed separation procedure

For isolation of target cells, non-B cells are labeled with a cocktail of biotinylated CD3ε, CD4, CD8a, CD49b, Gr-1, and Ter119 antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. Highly pure B cells are obtained by depletion of the magnetically labeled cells. The kit does not contain CD43 or CD11b in the depletion cocktail which might be expressed on some malignant target cells. It is therefore perfectly suited to be used for isolation of B cells from different mouse models for human diseases, e.g. B cells from B-CLL mouse models.

Columns

MS, LS, XS, or autoMACS
®
Columns.

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