Clone:
REA1204
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
Notch 2, hN2

Extended validation for Notch2 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of Notch2 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with Notch2-PE (REA1204, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of Notch2 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with Notch2-PE (REA1204, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of Notch2 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with Notch2-PE (REA1204, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of Notch2 knockout cells. Wild type (red) and knockout cells (blue) were stained with Notch2-PE, Clone (REA1204). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of Notch2 knockout cells. Wild type (red) and knockout cells (blue) were stained with Notch2-PE, Clone (REA1204). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Notch2 Antibody, anti-human, REAfinity™

Overview

Clone REA1204 recognizes human Notch2, a member of the Notch receptor family. Upon binding its membrane-bound ligand of the Delta-like or Jagged family members (Jagged1, Jagged2, and Delta1), the Notch receptor undergoes two proteolytic cleavage steps eventually releasing the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, where it activates transcription within a multiprotein complex. The Notch signaling is essential for the physiologic development of natural killer (NK) cells. Notch2 is expressed on various hematopetic cells, including T cell precursors in the thymus and activated peripheral T cells. In addition, the altered expression of Notch2 in various leukemias, lymphomas, and cancers has been reported.
Additional information: Clone REA1204 displays negligible binding to Fc receptors.

Alternative names

Notch 2, hN2

Detailed product information

Technical specifications

CloneREA1204
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
african green monkey (
Chlorocebus aethiops
)
AntigenNotch2
Alternative names of antigenNotch 2, hN2
Molecular mass of antigen [kDa]263
Distribution of antigenT cells, tumor cells, lymphoma, leukemia cells, thymocytes, other
Entrez Gene ID4853
RRIDAB_2811423, AB_2811429, AB_2811424, AB_2811430, AB_2811425, AB_2811431, AB_2811426, AB_2811432, AB_2811427, AB_2811428

Resources for Notch2 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Notch2 Antibody, anti-human, REAfinity™

Publications

  1. Haraguchi, K. et al. (2009) Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15. J Immunol 182(10): 6168-6178
  2. Gray, G. E. et al. (1999) Human ligands of the Notch receptor. Am. J. Pathol. 154(3): 785-794

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