Clone:
REA357
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
AOS5, AOVD1, TAN-1, hN1, Mis6, N1, lin-12

Specifications for Notch1 Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA357 recognizes the human and mouse neurogenic locus notch homolog protein 1 (notch1) antigen, a 300 kDa single-pass type I membrane protein also known as translocation-associated notch protein (TAN-1). Following cleavage in the Golgi, notch forms a heterodimer composed of a 180–200 kDa disulfide-linked extracellular domain (NEXT), and a 120 kDa membrane-bound intracellular portion (NICD). Upon ligand binding the latter is cleaved to form the notch intracellular domain transcription factor. Notch signaling controls cell fate decisions and is involved in multiple cellular differentiation steps during embryonic development and in adult organisms. In the immune system, notch1 plays a non-redundant role for thymic T cell development. Notch1 is also expressed by peripheral T cells and upregulated upon T cell activation. Clone REA357 recognizes the intracellular domain of mouse and human notch1, but not mouse notch2, 3, or 4.
Additional information: Clone REA357 displays negligible binding to Fc receptors.

Alternative names

AOS5, AOVD1, TAN-1, hN1, Mis6, N1, lin-12

Detailed product information

Technical specifications

CloneREA357
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse
AntigenNotch1
Alternative names of antigenAOS5, AOVD1, TAN-1, hN1, Mis6, N1, lin-12
Molecular mass of antigen [kDa] 269
Distribution of antigenT cells
Entrez Gene ID18128
RRIDAB_2653038, AB_2653039, AB_2653040, AB_2653041

References for Notch1 Antibody, anti-human/mouse, REAfinity™

Publications

  1. Weng, A. P. et al. (2004) Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia. Science 306(5694): 269-271
  2. Gray, G. E. et al. (1999) Human ligands of the Notch receptor. Am. J. Pathol. 154(3): 785-794
  3. Arruga, F. et al. (2014) Functional impact of NOTCH1 mutations in chronic lymphocytic leukemia. Leukemia 28(5): 1060-1070

Related products for
Notch1 Antibody, anti-human/mouse, REAfinity™

3 products available