Clone:
REA1162
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
NKR-P1 40, CD161c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C

Extended validation for NK1.1 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1162
PK136++
694370+
Cells were incubated with an excess of purified unconjugated NK1.1 (REA1162) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for NK1.1 Antibody, anti-mouse, REAfinity™

Overview

Clone REA1162 recognizes the mouse NK1.1 antigen, a single-pass type II membrane protein also known as CD161 or killer cell lectin-like receptor subfamily B member 1. NK1.1 is expressed by natural killer cells and a subset of T cells in the mouse strains C57BL/6, FVB/N, and NZB, but not in the mouse strains AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. It plays a stimulatory role on natural killer cells cytotoxicity. Activation by cross-linking of the receptor induces Ca
2+
mobilization and interferon-gamma production.
Additional information: Clone REA1162 displays negligible binding to Fc receptors.

Alternative names

NKR-P1 40, CD161c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C

Detailed product information

Technical specifications

CloneREA1162
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenNK1.1
Alternative names of antigenNKR-P1 40, CD161c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C
Molecular mass of antigen [kDa]25
Distribution of antigenNK cells, T cells, NKT cells
Entrez Gene ID17059
RRIDAB_2752122, AB_2752120, AB_2752123, AB_2752124, AB_2752125, AB_2752126, AB_2752127, AB_2752121, AB_2752128

References for NK1.1 Antibody, anti-mouse, REAfinity™

Publications

  1. Giorda, R. et al. (1992) Genomic structure and strain-specific expression of the natural killer cell receptor NKR-P1. J. Immunol. 149(6): 1957-1963
  2. Stenström, M. et al. (2005) Natural killer T-cell populations in C57BL/6 and NK1.1 congenic BALB.NK mice-a novel thymic subset defined in BALB.NK mice. Immunology 114(3): 336-345
  3. Ruiz, A. L. et al. (2014)
    NK1.1
    +
    CD8
    +
    T cells escape TGF-β control and contribute to early microbial pathogen response.
    Nat Commun. 5: 5150

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