The Neonatal Cardiac Fibroblast Isolation Kit, mouse has been designed for the enrichment of cardiac fibroblasts from dissociated neonatal mouse hearts. The isolation of cardiac fibroblasts has been successfully tested using neonatal mouse hearts from postnatal day 0 to day 3 (P0– P3).

Data and images for Neonatal Cardiac Fibroblast Isolation Kit, mouse

Figures

Figure 1

Cardiac fibroblasts were isolated from P2 CD-1R mouse hearts by using the Neonatal Cardiac Fibroblast Isolation Kit, two MS Columns, and a MiniMACS™ Separator. The cells were fluorescently stained with CD90.2-APC (# 130-091-790) and CD31-PE (# 130-097-422) and analyzed by flow cytometry using the MACSQuantR Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
After separation
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Figure 1

Cardiac fibroblasts were isolated from P2 CD-1R mouse hearts by using the Neonatal Cardiac Fibroblast Isolation Kit, two MS Columns, and a MiniMACS™ Separator. The cells were fluorescently stained with CD90.2-APC (# 130-091-790) and CD31-PE (# 130-097-422) and analyzed by flow cytometry using the MACSQuantR Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Cardiac fibroblasts were isolated from P2 CD-1R mouse hearts by using the Neonatal Cardiac Fibroblast Isolation Kit, two MS Columns, and a MiniMACS™ Separator. The cells were fluorescently stained with CD90.2-APC (# 130-091-790) and CD31-PE (# 130-097-422) and analyzed by flow cytometry using the MACSQuantR Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Neonatal Cardiac Fibroblast Isolation Kit, mouse

Overview

The Neonatal Cardiac Fibroblast Isolation Kit, mouse has been designed for the enrichment of cardiac fibroblasts from dissociated neonatal mouse hearts. The isolation of cardiac fibroblasts has been successfully tested using neonatal mouse hearts from postnatal day 0 to day 3 (P0– P3).

Detailed product information

Background information

Cardiac fibroblasts are one of the most frequent cell types within the heart and play a critical role (i) in heart development, (ii) in maintaining normal cardiac function, as well as (iii) in cardiac remodeling during pathological conditions such as myocardial infarction.The Neonatal Cardiac Fibroblast Isolation Kit, mouse has been designed for the enrichment of cardiac fibroblasts from dissociated neonatal mouse hearts. The isolation of cardiac fibroblasts has been successfully tested using neonatal mouse hearts from postnatal day 0 to day 3 (P0– P3). For optimal results, the Neonatal Cardiac Fibroblast Isolation Kit should be used in combination with the Neonatal Heart Dissociation Kit, mouse and rat (# 130-098-373).

Applications

  • Enrichment of cardiac fibroblasts from neonatal (P0-P3) mouse hearts.
  • Culture and expansion or direct use of enriched cardiac fibroblasts for biochemical, physiological and pharmacological studies.

Columns

MS or autoMACS
®
Columns.

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