Clone:
REA399
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
MYH6, MYH7, MHC, MYHC

Specifications for Myosin Heavy Chain Antibody, anti-human/mouse/rat, REAfinity™

Overview

Clone REA399 recognizes the human, mouse, and rat cardiac isoform of the myosin heavy chain (MHC) antigen. Myosin forms cross-bridges with actin in cardiac, smooth, and skeletal muscle, and cyclic cross-bridge activity is the basis for muscular contraction. It is a hexamer composed of two heavy chains, each of which contains an actin-binding site and an ATP hydrolysis site, and four light chains. Isoforms of each myosin subunit are expressed in cardiac, skeletal, and smooth muscle, and many are associated with distinct contractile properties in the muscles or single muscle fibers in which they are expressed. In cardiac muscle, two MHC isoforms, termed α-MHC and β-MHC, are present. α-MHC is associated with higher actomyosin ATPase activity compared with β-MHC. The relative amounts of the two isoforms may vary, with the ratio being a function of the hormonal status, the developmental stage, the animal, or the particular region of the heart.
Additional information: Clone REA399 displays negligible binding to Fc receptors.

Alternative names

MYH6, MYH7, MHC, MYHC

Detailed product information

Technical specifications

CloneREA399
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse, rat, other
Cross-reactivityrabbit, cow, dog, pig
AntigenMyosin Heavy Chain
Alternative names of antigenMYH6, MYH7, MHC, MYHC
Molecular mass of antigen [kDa]223(average of subunits)
Distribution of antigencardiac muscle, skeletal muscle, smooth muscle
Entrez Gene ID4624, 4625
RRIDAB_2652979, AB_2652980, AB_2652981

References for Myosin Heavy Chain Antibody, anti-human/mouse/rat, REAfinity™

Publications

  1. Cosper, P. F. et al. (2012) Interferon-γ causes cardiac myocyte atrophy via selective degradation of myosin heavy chain in a model of chronic myocarditis. Am. J. Pathol. 181(6): 2038-2046
  2. Haddad, F. et al. (2010) Cardiac myosin heavy chain gene regulation by thyroid hormone involves altered histone modifications. Am. J. Physiol. Heart Circ. Physiol. 299(6): H1968-H1980
  3. Molkentin, J. D. et al. (1996) Alpha-myosin heavy chain gene regulation: delineation and characterization of the cardiac muscle-specific enhancer and muscle-specific promoter. J. Mol. Cell. Cardiol. 28(6): 1211-1225

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