Clone:
REA491
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS, IF, IHC, ICC, MC
Alternative names:
Myeloperoxidase

Specifications for MPO Antibody, anti-human, REAfinity™

Overview

Clone REA491 recognizes the human myeloperoxidase (MPO) antigen, a heterotetrameric protein consisting of two 15 kDa light chains and two variable-weight glycosylated heavy chains bound to a prosthetic heme group. MPO produces hypochlorous acid from hydrogen peroxide and chloride anion during the neutrophil's respiratory burst. Furthermore, it oxidizes tyrosine to tyrosyl radical using hydrogen peroxide as an oxidizing agent. MPO is present in azurophilic granules of neutrophilic granulocytes and monocytes and is also expressed in some acute myeloid leukemia cells. It plays a major role in the oxygen-dependent microbicidal system of of polymorphonuclear leukocytes. The deficiency of this enzyme activity results in a marked microbicidal defect.
Additional information: Clone REA491 displays negligible binding to Fc receptors.

Alternative names

Myeloperoxidase

Detailed product information

Technical specifications

CloneREA491
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenMPO
Alternative names of antigenMyeloperoxidase
Molecular mass of antigen [kDa]66(sum of molecular weights of subunits)
Distribution of antigengranulocytes, monocytes, neutrophils
Entrez Gene ID4353
RRIDAB_2784435, AB_2752013, AB_2751983, AB_2751873, AB_2751843, AB_2819418, AB_2784436

References for MPO Antibody, anti-human, REAfinity™

Publications

  1. Adair, J. E. et al. (2018) Novel lineage depletion preserves autologous blood stem cells for gene therapy of Fanconi anemia complementation group A. Haematologica 103(11): 1806-1814
  2. Manivannan, P. et al. (2014) Can threshold for MPO by flow cytometry be reduced in classifying acute leukaemia? A comparison of flow cytometric and cytochemical myeloperoxidase using different flow cytometric cut-offs. Haematologica : Epub ahead of print
  3. Morishita, K. et al. (1987) Molecular cloning and characterization of cDNA for human myeloperoxidase. J. Biol. Chem. 262(8): 3844-3851
  4. Papayannopoulos, V. et al. (2010) Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps. J. Cell Biol. 191(3): 677-697

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