IL-23 stands for interleukin 23. Mouse IL-23 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Mouse IL-23

Figures

Figure 1

View details
Mouse IL-23 activity assay.
The biological activity of Mouse IL-23 was determined by assessment of IL-17 secretion by mouse splenocytes activated with 10 ng/mL PMA.

Figure 1

Mouse IL-23 activity assay.
The biological activity of Mouse IL-23 was determined by assessment of IL-17 secretion by mouse splenocytes activated with 10 ng/mL PMA.

Figure 2

View details
SDS-PAGE of Mouse IL-23
under reducing conditions (R). Units p40 and p19 are fused by a flexible linker region resulting in a single-chain polypeptide. The theoretical molecular mass is 58 kDa. Due to glycosylation the protein has an apparent mass of 70 kDa.

Figure 2

SDS-PAGE of Mouse IL-23
under reducing conditions (R). Units p40 and p19 are fused by a flexible linker region resulting in a single-chain polypeptide. The theoretical molecular mass is 58 kDa. Due to glycosylation the protein has an apparent mass of 70 kDa.

Specifications for Mouse IL-23

Overview

IL-23 stands for interleukin 23. Mouse IL-23 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-23 can be used for a variety of applications, including:
  • In vitro differentiation of CD4+ T cells towards TH17 cells and proliferation of TH17 cells.
  • Exploration of the role of IL-23 in autoimmune diseases or inflammatory bowel diseases.
  • Investigation of IL-23-mediated molecular signaling pathways.

Detailed product information

Background information

IL-23 is a covalently linked heterodimeric cytokine, which is closely related to IL-12. Macrophages and dendritic cells are the major sources of both IL-12 and IL-23, which act on receptors primarily expressed by T cells, NK cells, and NKT cells. IL-23 consists of the p40 subunit shared with IL-12 and the 19 kDa protein p19 that is unique to IL-23. Its effects are mediated by a receptor composed of IL-12 receptor subunit β1 and an IL-23 specific subunit. IL-12 and IL-23 show different functions although both contain the p40 subunit. While IL-12 drives the development of T
H
1 cells, IL-23 mediates the full differentiation and maintenance of T
H
17 cells. IL-23 stimulates CD4
+
T cells and promotes the production of pro-inflammatory cytokines. As a key cytokine in the survival and proliferation of T
H
17 cells, IL-23 has central roles in autoimmune diseases and promotes chronic inflammation. It has been shown that single chain fusion proteins of naturally heterodimeric cytokines such as IL-12 or IL-23 are bioactive
in vitro
and
in vivo
.

Biological activity

  • Induction of IL-17 secretion by mouse splenocytes
  • research grade: ≥ 4×
    10
    6
    U/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Resources for Mouse IL-23

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Mouse IL-23

Publications

  1. Damsker, J.M. et al. (2010) Tʜ1 and Tʜ17 cells: adversaries and collaborators. Ann. N. Y. Acad. Sci. 1183: 211-221
  2. Lieschke, G.J. et al. (1997)
    Bioactive murine and human interleukin-12 fusion proteins which retain antitumor activity
    in vivo.
    Nat. Biotechnol. 15: 35-40
  3. Miller, J.M. et al. (2010)
    Vesicular stomatitis virus modified with single chain IL-23 exhibits oncolytic activity against tumor cells
    in vitro
    and
    in vivo.
    Int. J. Infereron Cytokine Mediator Res. 2010: 63-72
  4. Aggarwal, S. et al. (2003) Interleukin-23 promotes a distinct CD4 T cell activation state characterized by the production of interleukin-17. J. Biol. Chem. 278: 1910-1914

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