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BALB/c mice were immunized i.p. with hen eggwhite lysozyme (HEL) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with HEL for 15 hours. The responding cells were stained and enriched according to secretion of IFN-γ using the Mouse IFN-γ Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 693 IFN-γ- secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 67 IFN-γ-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
Stimulated sample |
Before enrichment | After enrichment |
Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized i.p. with hen eggwhite lysozyme (HEL) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with HEL for 15 hours. The responding cells were stained and enriched according to secretion of IFN-γ using the Mouse IFN-γ Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 693 IFN-γ- secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 67 IFN-γ-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. | Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized i.p. with hen eggwhite lysozyme (HEL) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with HEL for 15 hours. The responding cells were stained and enriched according to secretion of IFN-γ using the Mouse IFN-γ Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 693 IFN-γ- secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 67 IFN-γ-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
Unstimulated control |
Before enrichment | After enrichment |
Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized i.p. with hen eggwhite lysozyme (HEL) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with HEL for 15 hours. The responding cells were stained and enriched according to secretion of IFN-γ using the Mouse IFN-γ Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 693 IFN-γ- secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 67 IFN-γ-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. | Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized i.p. with hen eggwhite lysozyme (HEL) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with HEL for 15 hours. The responding cells were stained and enriched according to secretion of IFN-γ using the Mouse IFN-γ Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 693 IFN-γ- secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 67 IFN-γ-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
* Percentage represents frequency among CD4+ T cells. |
** Percentage represents frequency among enriched cells. |
In this example, BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for co-expression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (APC) and Mouse IL-2 Secretion Assay - Detection Kit (PE). The plots show co-expression of IFN-γ- and IL-2-secreting cells gated on viable CD4 + T cells on the stimulated sample as well as on the unstimulated control. |
Stimulated sample | Unstimulated control |
Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 2In this example, BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for co-expression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (APC) and Mouse IL-2 Secretion Assay - Detection Kit (PE). The plots show co-expression of IFN-γ- and IL-2-secreting cells gated on viable CD4 + T cells on the stimulated sample as well as on the unstimulated control. | Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 2In this example, BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for co-expression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (APC) and Mouse IL-2 Secretion Assay - Detection Kit (PE). The plots show co-expression of IFN-γ- and IL-2-secreting cells gated on viable CD4 + T cells on the stimulated sample as well as on the unstimulated control. |
* Percentage represents frequency among CD4+ T cells. |
BALB/c mice were immunized i.p. with hen eggwhite lysozyme (HEL) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with HEL for 15 hours. The responding cells were stained and enriched according to secretion of IFN-γ using the Mouse IFN-γ Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 693 IFN-γ- secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 67 IFN-γ-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
Stimulated sample |
Before enrichment | After enrichment |
Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized i.p. with hen eggwhite lysozyme (HEL) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with HEL for 15 hours. The responding cells were stained and enriched according to secretion of IFN-γ using the Mouse IFN-γ Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 693 IFN-γ- secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 67 IFN-γ-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. | Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized i.p. with hen eggwhite lysozyme (HEL) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with HEL for 15 hours. The responding cells were stained and enriched according to secretion of IFN-γ using the Mouse IFN-γ Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 693 IFN-γ- secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 67 IFN-γ-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
Unstimulated control |
Before enrichment | After enrichment |
Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized i.p. with hen eggwhite lysozyme (HEL) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with HEL for 15 hours. The responding cells were stained and enriched according to secretion of IFN-γ using the Mouse IFN-γ Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 693 IFN-γ- secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 67 IFN-γ-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. | Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 1BALB/c mice were immunized i.p. with hen eggwhite lysozyme (HEL) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with HEL for 15 hours. The responding cells were stained and enriched according to secretion of IFN-γ using the Mouse IFN-γ Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 693 IFN-γ- secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 67 IFN-γ-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
* Percentage represents frequency among CD4+ T cells. |
** Percentage represents frequency among enriched cells. |
In this example, BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for co-expression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (APC) and Mouse IL-2 Secretion Assay - Detection Kit (PE). The plots show co-expression of IFN-γ- and IL-2-secreting cells gated on viable CD4 + T cells on the stimulated sample as well as on the unstimulated control. |
Stimulated sample | Unstimulated control |
Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 2In this example, BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for co-expression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (APC) and Mouse IL-2 Secretion Assay - Detection Kit (PE). The plots show co-expression of IFN-γ- and IL-2-secreting cells gated on viable CD4 + T cells on the stimulated sample as well as on the unstimulated control. | Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)Figure 2In this example, BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for co-expression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (APC) and Mouse IL-2 Secretion Assay - Detection Kit (PE). The plots show co-expression of IFN-γ- and IL-2-secreting cells gated on viable CD4 + T cells on the stimulated sample as well as on the unstimulated control. |
* Percentage represents frequency among CD4+ T cells. |
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