The Monocyte Isolation Kit (BM) has been developed for the easy isolation of monocytes from mouse bone marrow by depletion of unwanted cells.

Data and images for Monocyte Isolation Kit (BM), mouse

Figures

Figure 1

Monocytes were isolated from C57BL/6 bone marrow cells by using the Monocyte Isolation Kit (BM), mouse, an LS Column and a QuadroMACS Separator. The cells were fluorescently stained with CD11b-VioBlue, Lin cocktail (CD3ε-PE, CD45R (B220)-PE, Anti-NK1.1-PE, Anti-Ly-6G-PE, CD49b-PE), Anti-Ly-6C-FITC and Anti-Siglec-F-APC and analyzed by flow cytometry using the MACSQuant Analyser. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
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Figure 1

Monocytes were isolated from C57BL/6 bone marrow cells by using the Monocyte Isolation Kit (BM), mouse, an LS Column and a QuadroMACS Separator. The cells were fluorescently stained with CD11b-VioBlue, Lin cocktail (CD3ε-PE, CD45R (B220)-PE, Anti-NK1.1-PE, Anti-Ly-6G-PE, CD49b-PE), Anti-Ly-6C-FITC and Anti-Siglec-F-APC and analyzed by flow cytometry using the MACSQuant Analyser. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Monocytes were isolated from C57BL/6 bone marrow cells by using the Monocyte Isolation Kit (BM), mouse, an LS Column and a QuadroMACS Separator. The cells were fluorescently stained with CD11b-VioBlue, Lin cocktail (CD3ε-PE, CD45R (B220)-PE, Anti-NK1.1-PE, Anti-Ly-6G-PE, CD49b-PE), Anti-Ly-6C-FITC and Anti-Siglec-F-APC and analyzed by flow cytometry using the MACSQuant Analyser. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Isolated monocytes
View details

Figure 1

Monocytes were isolated from C57BL/6 bone marrow cells by using the Monocyte Isolation Kit (BM), mouse, an LS Column and a QuadroMACS Separator. The cells were fluorescently stained with CD11b-VioBlue, Lin cocktail (CD3ε-PE, CD45R (B220)-PE, Anti-NK1.1-PE, Anti-Ly-6G-PE, CD49b-PE), Anti-Ly-6C-FITC and Anti-Siglec-F-APC and analyzed by flow cytometry using the MACSQuant Analyser. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Monocytes were isolated from C57BL/6 bone marrow cells by using the Monocyte Isolation Kit (BM), mouse, an LS Column and a QuadroMACS Separator. The cells were fluorescently stained with CD11b-VioBlue, Lin cocktail (CD3ε-PE, CD45R (B220)-PE, Anti-NK1.1-PE, Anti-Ly-6G-PE, CD49b-PE), Anti-Ly-6C-FITC and Anti-Siglec-F-APC and analyzed by flow cytometry using the MACSQuant Analyser. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Monocyte Isolation Kit (BM), mouse

Overview

The Monocyte Isolation Kit (BM) has been developed for the easy isolation of monocytes from mouse bone marrow by depletion of unwanted cells.

Detailed product information

Detailed separation procedure

Using the Monocyte Isolation Kit (BM), mouse, monocytes are isolated by depletion of non-target cells. Non-target cells are indirectly magnetically labeled with a cocktail of biotin-conjugated monoclonal antibodies, as primary labeling reagent, and anti-biotin monoclonal antibodies conjugated to MicroBeads as secondary labeling reagent. The unwanted cells are depleted by retaining them within a MACS Column in the magnetic field of a MACS Separator, while the unlabeled monocytes pass through the column.

Downstream applications

Isolation of mouse monocytes from mouse bone marrow for in vitro analysis, differentiation studies, co-culture or adoptive transfer experiments.

Columns

LS or autoMACS
®
Columns.

Reviews for Monocyte Isolation Kit (BM), mouse

Clean Monocyte Isolation

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Monocyte Isolation Kit (BM), mouse (130-100-629)

Monocytes were isolated from a spleen from a wildtype B6 mouse. Single cells were isolated and monocytes were isolated per manufacturer's protocol.

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