The Mo-DC Differentiation Inspector is designed for the easy and rapid evaluation of the
in vitro
generation of Mo-DCs.

Data and images for Mo-DC Differentiation Inspector, human

Figures

Figure 1

Flow cytometric analysis of in vitro generated monocyte-derived dendritic cells (Mo-DCs) using the Mo-DC Differentiation Inspector. Monocytes isolated with CD14 MicroBeads from human PBMCs were differentiated into Mo-DCs in the presence of GM-CSF and IL-4 for 7 days using the Mo-DC Differentiation Medium. Immature Mo-DCs were differentiated into mature Mo-DCs using the Mo-DC Maturation Medium containing TNF-α. Shown are the expression levels of CD14 (A), CD209 (DC-SIGN) (B), and CD83 (C) as mean fluorescence intensities (MFI) on monocytes, immature Mo-DCs (imMo-DCs), and mature Mo-DCs (mMo-DCs) prepared from 12 donors.
A:
B:
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Figure 1

Flow cytometric analysis of in vitro generated monocyte-derived dendritic cells (Mo-DCs) using the Mo-DC Differentiation Inspector. Monocytes isolated with CD14 MicroBeads from human PBMCs were differentiated into Mo-DCs in the presence of GM-CSF and IL-4 for 7 days using the Mo-DC Differentiation Medium. Immature Mo-DCs were differentiated into mature Mo-DCs using the Mo-DC Maturation Medium containing TNF-α. Shown are the expression levels of CD14 (A), CD209 (DC-SIGN) (B), and CD83 (C) as mean fluorescence intensities (MFI) on monocytes, immature Mo-DCs (imMo-DCs), and mature Mo-DCs (mMo-DCs) prepared from 12 donors.
View details

Figure 1

Flow cytometric analysis of in vitro generated monocyte-derived dendritic cells (Mo-DCs) using the Mo-DC Differentiation Inspector. Monocytes isolated with CD14 MicroBeads from human PBMCs were differentiated into Mo-DCs in the presence of GM-CSF and IL-4 for 7 days using the Mo-DC Differentiation Medium. Immature Mo-DCs were differentiated into mature Mo-DCs using the Mo-DC Maturation Medium containing TNF-α. Shown are the expression levels of CD14 (A), CD209 (DC-SIGN) (B), and CD83 (C) as mean fluorescence intensities (MFI) on monocytes, immature Mo-DCs (imMo-DCs), and mature Mo-DCs (mMo-DCs) prepared from 12 donors.
C:
View details

Figure 1

Flow cytometric analysis of in vitro generated monocyte-derived dendritic cells (Mo-DCs) using the Mo-DC Differentiation Inspector. Monocytes isolated with CD14 MicroBeads from human PBMCs were differentiated into Mo-DCs in the presence of GM-CSF and IL-4 for 7 days using the Mo-DC Differentiation Medium. Immature Mo-DCs were differentiated into mature Mo-DCs using the Mo-DC Maturation Medium containing TNF-α. Shown are the expression levels of CD14 (A), CD209 (DC-SIGN) (B), and CD83 (C) as mean fluorescence intensities (MFI) on monocytes, immature Mo-DCs (imMo-DCs), and mature Mo-DCs (mMo-DCs) prepared from 12 donors.

Specifications for Mo-DC Differentiation Inspector, human

Overview

The Mo-DC Differentiation Inspector is designed for the easy and rapid evaluation of the
in vitro
generation of Mo-DCs.

Detailed product information

Background information

The use of the pre-mixed Mo-DC Differentiation Inspector Cocktail minimizes pipetting steps. All staining antibodies included in the cocktail are optimally titrated and simply added to the separated or differentiated monocytes in a single step before flow cytometry. The Mo-DC Differentiation Inspector comprises monoclonal antibodies recognizing CD14, CD83, and CD209 and corresponding isotype controls.

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