The Memory CD4
+
T Cell Isolation Kit was developed for the isolation of untouched memory T helper cells from human peripheral blood.

Data and images for
Memory CD4
+
T Cell Isolation Kit
, human

Figures

Figure 1

Isolation of untouched memory CD4
+
T helper cells from PBMCs using the Memory CD4
+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator.
PBMCs before separation
Untouched CD4
+
memory T cells
View details

Figure 1

Isolation of untouched memory CD4
+
T helper cells from PBMCs using the Memory CD4
+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator.
View details

Figure 1

Isolation of untouched memory CD4
+
T helper cells from PBMCs using the Memory CD4
+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator.

Specifications for
Memory CD4
+
T Cell Isolation Kit
, human

Overview

The Memory CD4
+
T Cell Isolation Kit was developed for the isolation of untouched memory T helper cells from human peripheral blood.

Detailed product information

Detailed separation procedure

For magnetic depletion of naive T cells, CD8
+
T cells, B cells, NK cells, γ/δ T cells, monocytes, DCs, granulocytes, platelets, and erythroid cells, PBMCs are incubated with a cocktail of biotinylated CD45RA, CD8, CD14, CD16, CD19, CD56, CD36, CD123, anti-TCRγ/δ, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. The Memory CD4
+
T Cell Isolation Kit provides a fast and efficient way to isolate untouched memory CD4
+
T cells.

Downstream applications

CD4
+
memory T cells isolated with the Memory CD4
+
T Cell Isolation Kit are used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on memory T helper cell activation. Untouched memory CD4
+
T cells can also be used for further isolation of specific subsets, such as CCR8
+/–
cells
1
.

Columns

MS, LS, or autoMACS
®
Columns.

References for
Memory CD4
+
T Cell Isolation Kit
, human

Publications

  1. Soler, D. et al. (2006) CCR8 expression identifies CD4 memory T cells enriched for FOXP3+ regulatory and Th2 effector lymphocytes. J. Immunol. 177: 6940-6951

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Memory CD4
+
T Cell Isolation Kit
, human

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