The MACSxpress
®
Buffy Coat NK Cell Isolation Kit was developed for the isolation of untouched natural killer (NK) cells from buffy coat without density gradient centrifugation.

Data and images for
MACSxpress
®
Buffy Coat NK Cell Isolation Kit
, human

Figures

Figure 1

Untouched NK cells were isolated from 10 mL of buffy coat using the MACSxpress
®
Buffy Coat NK Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with Propidium Iodide Solution, CD45-VioGreen™, CD3-VioBlue
®
, and CD56-APC-Vio
®
770 and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10.
Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
Before separation
After separation
View details

Figure 1

Untouched NK cells were isolated from 10 mL of buffy coat using the MACSxpress
®
Buffy Coat NK Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with Propidium Iodide Solution, CD45-VioGreen™, CD3-VioBlue
®
, and CD56-APC-Vio
®
770 and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10.
Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
View details

Figure 1

Untouched NK cells were isolated from 10 mL of buffy coat using the MACSxpress
®
Buffy Coat NK Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with Propidium Iodide Solution, CD45-VioGreen™, CD3-VioBlue
®
, and CD56-APC-Vio
®
770 and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10.
Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Specifications for
MACSxpress
®
Buffy Coat NK Cell Isolation Kit
, human

Overview

The MACSxpress
®
Buffy Coat NK Cell Isolation Kit was developed for the isolation of untouched natural killer (NK) cells from buffy coat without density gradient centrifugation.

Detailed product information

Detailed separation procedure

With the new MACSxpress
®
Technology, untouched human leukocyte subsets can be isolated from up to 30 mL of buffy coat. Non-target cells are removed by immunomagnetic depletion using MACSxpress Beads. Simultaneously, erythrocytes are sedimented, yielding untouched target cells of high purity.

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®
Buffy Coat NK Cell Isolation Kit
, human

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