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Mouse whole brain was dissociated using the Neural Tissue Dissociation Kit (P) and the gentleMACS™ Dissociator. Neural stem cells (NSCs) were isolated via Anti-Prominin-1 MicroBeads and subsequently cultivated in MACS® Neuro Medium supplemented with MACS NeuroBrew-21, penicillin/streptomycin, 2 mM L-glutamine, 20 ng/mL human EGF and FGF-2. A) Primary neurospheres formed by the NSCs after one week in culture. B) Single-cell suspension after dissociation of primary neurospheres using the Neurosphere Dissociation Kit (P). C) Secondary neurospheres formed one week after dissociation.