Embryoid Body Dissociation Kit, human and mouse

Embryoid Body Dissociation Kit, human and mouse

The Embryoid Body Dissociation Kit was developed for standardized and reproducible dissociation of
in vitro
generated EBs or pluripotent stem cell-derived neurospheres. This convenient and time saving kit was optimized for high yields, high cell viability and high reproducibility.

Background information

Embryoid Body (EB) formation is a crucial step in many ES or iPS cell differentiation protocols. Viable single-cell suspensions of EBs are a prerequisite for subsequent cell analysis or isolation of specific cell populations within the EB.

Downstream applications

  • Isolation of EB-derived cell populations using e.g. MACS Cell Separation Technology
  • Cultivation of EB-derived cells
  • Phenotyping or enumeration of individual EB-derived cell populations by flow cytometry
  • Selected references

    1. Niwa, A. et al. (2011) A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors. PLoS One 6: e22261
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