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Samples are excited from the side by a focused light sheet while the fluorescence light is detected by a sCMOS camera perpendicular to the illumination plane. 3D image stacks are generated by moving the sample through the light sheet.
High-speed imaging of fragile life specimens and large cleared tissue
Separated illumination (excitation) and detection (emission) beam paths.
As only the actually observed section is illuminated, photodamage and fluorophore bleaching are kept at a minimum.
Minimum photodamage and fluorophore bleaching
Formation of murine lymph vessels: Progenitor cells (pink), aorta (green), vain (blue) [Courtesy of Friedemann Kiefer, René Hägerling, Cathrin Dierkes, European Institute for Molecular Imaging (EIMI), Münster]