Interleukin 2 (IL-2), a potent lymphoid cell growth factor, plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes, for instance, proliferation and differentiation of T cells, NK cells, and B cells.
MACS GMP Recombinant Human IL-2 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Data and images for
MACS
®
GMP Recombinant Human IL-2

Figures

Figure 1

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Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-2. The peak corresponds to the calculated molecular mass of 15400 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-2. The peak corresponds to the calculated molecular mass of 15400 Da.

Figure 2

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MACS GMP Recombinant Human IL-2 biological activity.
Activity of Human IL-2, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 2

MACS GMP Recombinant Human IL-2 biological activity.
Activity of Human IL-2, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 3

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MACS GMP Recombinant Human IL-2 batch-to-batch variability.
Activity of three different production batches of Human MACS GMP IL-2.

Figure 3

MACS GMP Recombinant Human IL-2 batch-to-batch variability.
Activity of three different production batches of Human MACS GMP IL-2.

Specifications for
MACS
®
GMP Recombinant Human IL-2

Overview

Interleukin 2 (IL-2), a potent lymphoid cell growth factor, plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes, for instance, proliferation and differentiation of T cells, NK cells, and B cells.
MACS GMP Recombinant Human IL-2 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

IL-2 is a typical four α-helix bundle cytokine and is produced by activated T cells, especially the CD4
+
T helper cell population. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β-chain, and common γ-chain.

Applications

MACS GMP Recombinant Human IL-2 can be used for a variety of applications, including the
ex vivo
activation and expansion of T cells, e.g., antigen-specific cytotoxic T lymphocytes
2,3
or regulatory T cells
4
or the
ex vivo
stimulation of NK cells
5,6
.

Biological activity

  • The specific activity is determined by proliferation assay according to Gearing and Bird
    1
    using CTLL-2 cells. The proliferation assay was calibrated with the 2nd international standard for human IL-2 (NIBSC code 86/500) provided by the National Institute for Biological Standards and Control.
  • Specific activity: ≥ 1×
    10
    6
    IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Resources for
MACS
®
GMP Recombinant Human IL-2

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for
MACS
®
GMP Recombinant Human IL-2

Publications

  1. Gearing, A. J. H. and Bird, C. B. (1987) Lymphokines and interferons, a practical approach. Oxford: IRL Press : 295
  2. Zhang, H. et al. (2007) 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy. J. Immunol. 179: 4910-4918
  3. Hinrichs et al. (2008)
    IL-2 and IL-21 confer opposing differentiation programs to CD8
    +
    T cells for adoptive immunotherapy.
    Blood 111: 5326-5333
  4. Peters, J. H. et al. (2008)
    Ex vivo
    generation of human alloantigen-specific regulatory T cells from CD4
    +
    CD25
    high
    T cells for immunotherapy.
    PLoS One 3(5): e2233
  5. Berg et al. (2009)
    Clinical-grade
    ex vivo
    -expanded human natural killer cells up-regulate activating receptors and death receptor ligands and have enhanced cytolytic activity against tumor cells.
    Cytotherapy 11: 341-355
  6. McKenna, D. H. Jr. et al. (2007) Good manufacturing practices production of natural killer cells for immunotherapy: a six-year single-institution experience. Transfusion 47(3): 520-528

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®
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